Anti-EAAT2/SLC1A2 Polyclonal Antibody for WB, IF-P, ELISA - P43004

Core Product Specifications and Parameters

Biological Background: EAAT2/SLC1A2 Function and Localization

• EAAT2 (Excitatory Amino Acid Transporter 2), also designated GLT-1 and SLC1A2, is a member of the solute carrier family 1 (SLC1) that mediates high-affinity glutamate transport.
• It functions as a sodium-dependent symporter, mediating the uptake of L-glutamate, L-aspartate, and D-aspartate alongside 2–3 Na+ ions and one H+, with counter-transport of one K+ ion. Related references: PMID:14506254 PMID:15265858 PMID:26690923
• Essential for the rapid removal of synaptically released glutamate, EAAT2 terminates excitatory signaling and prevents excitotoxic neuronal damage.
• The transporter also conducts a chloride flux uncoupled from amino acid transport, contributing to charge balance during operation. Related references: PMID:14506254
• Predominantly localized to the plasma membrane, EAAT2 expression is enriched in astrocytes throughout the central nervous system.
• Alternative splicing generates multiple isoforms, and post-translational modifications including phosphorylation, palmitoylation, and N-linked glycosylation regulate its trafficking and activity.
• Mutations in SLC1A2 are associated with epileptic encephalopathy, underscoring its critical role in neurological health.

Experimental Guidance and Technical Tips

• The immunogen spans residues 474–574 within the intracellular C-terminal domain, a region that exhibits moderate cross-species conservation; this likely accounts for the antibody’s reactivity with both mouse and rat orthologues.
• For Western blotting, the predicted mass is 62 kDa; however, glycosylation and other modifications may result in observed bands at higher molecular weights. Include appropriate positive and negative controls.
• In immunofluorescence protocols, permeabilization is required to access the intracellular epitope. Co-staining with a plasma membrane marker is advisable to confirm localization.
• ELISA applications can be adapted for quantification of EAAT2 in tissue lysates or biological fluids; optimize blocking and detection conditions for your specific sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a single-source partner for IVD raw materials, connecting diagnostic developers with validated antibodies like this EAAT2/SLC1A2 rabbit pAb for neuroscience and transporter-focused research. Our portfolio encompasses validated antibody pairs, bulk monoclonals and polyclonals, and ancillary reagents, complemented by sourcing capabilities for rare-target raw materials—supporting your assay pipeline from initial design through clinical application.