Anti-EAAT2 Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P43004

Core Product Specifications and Parameters

Biological Background: EAAT2 Function and Localization

• EAAT2 is a sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate, L-aspartate, and D-aspartate. Related references: PMID:14506254 PMID:15265858 PMID:26690923
• It functions as a symporter, co-transporting one amino acid molecule with two or three Na⁺ ions and one proton, while counter-transporting one K⁺ ion. Related references: PMID:14506254
• EAAT2 also mediates a chloride flux that is not coupled to amino acid transport, which helps prevent charge imbalance. Related references: PMID:14506254
• The transporter is essential for rapid clearance of glutamate from the synaptic cleft, terminating postsynaptic excitation.
• EAAT2 is a multi-pass membrane protein localized to the cell membrane.
• It undergoes glycosylation, palmitoylation, and phosphorylation; these modifications may regulate its activity and trafficking.
• Alternative splicing produces several isoforms, and mutations in SLC1A2 are linked to epileptic encephalopathies (reflected in aliases DEE41 and EIEE41).
• The canonical isoform has a molecular weight of ~62 kDa.

Experimental Guidance and Technical Tips

• For western blotting, the predicted molecular weight is ~62 kDa; validate with appropriate positive and negative controls.
• For immunofluorescence and immunohistochemistry, consider fixation/permeabilization conditions to preserve membrane protein accessibility.
• The antibody cross-reacts with mouse and rat, making it suitable for translational studies; verify in the respective species using appropriate tissue lysates.
• In ELISA, use a carefully optimized coating buffer and blocking agent to minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This EAAT2 monoclonal antibody supports neuroscience and neurological disease research, particularly glutamate transporter and synaptic function studies. We also offer validated antibody pairs, bulk ancillary reagents, and rare target sourcing to accelerate your diagnostic development.