Anti-E2F8 Polyclonal Antibody for WB and ELISA - A0AVK6

Core Product Specifications and Parameters

Biological Background: Transcription factor E2F8 Function and Localization

• Atypical E2F transcription factor that mainly acts as a transcription repressor, binding DNA independently of DP proteins and recognizing the E2 recognition site 5'-TTTC[CG]CGC-3'.
• Directly represses transcription of classical E2F transcription factors such as E2F1, forming a feedback loop in S phase to prevent p53/TP53-dependent apoptosis.
• Plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, repressing genes promoting cytokinesis and antagonizing classical E2F proteins (E2F1, E2F2, and/or E2F3).
• Required for placental development by promoting polyploidization of trophoblast giant cells.
• Acts as a promoter of sprouting angiogenesis, possibly as a transcription activator, associating with HIF1A, binding the VEGFA promoter and activating VEGFA expression.
• Located in the nucleus.
• Additional keywords: 3D-structure, Activator, Cell cycle, DNA-binding, Nucleus, Phosphoprotein, Repressor, Transcription, Transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 788-867) of human E2F8, providing target specificity. Ensure antibody recognition is confirmed in relevant cell or tissue lysates.
• For Western blot, expect a band near the predicted molecular weight of ~94 kDa (UniProt: 94.2 kDa), though post-translational modifications like phosphorylation may cause shifts.
• For ELISA, use recombinant E2F8 protein or the specific immunogen peptide as coating antigen for optimal detection.
• Cross-reactivity with human and mouse is reported; validate in your specific sample system and consider sequence alignment for other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing solution for IVD raw materials, supporting research on transcription factors like E2F8 involved in cell cycle and angiogenesis. We provide validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents, along with rare target raw-material sourcing to accelerate your diagnostic and research projects.