Anti-E2F4 Rabbit Monoclonal Antibody for WB and ELISA - Q16254

Core Product Specifications and Parameters

Biological Background: E2F4 Function and Localization

• E2F4 is a member of the E2F family of transcription factors, playing a crucial role in cell cycle regulation and transcriptional control.
• It binds DNA cooperatively with DP proteins at E2 recognition sites (5'-TTTC[CG]CGC-3') found in promoters of cell cycle and DNA replication genes.
• Functions as a transcription activator, driving the G1 to S phase transition and coordinating the expression of genes required for DNA synthesis.
• Binds with high affinity to pocket proteins RBL1 and RBL2, and can also interact with RB1 under certain conditions.
• Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, it activates genes essential for centriole biogenesis.
• Localizes to the nucleus, consistent with its role in transcriptional regulation.
• Widely expressed across human tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
• Undergoes post-translational modifications such as acetylation and phosphorylation, which modulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a synthetic peptide corresponding to amino acids 250–350 of human E2F4. For Western blot, anticipate a band around 44 kDa. Validate specificity in your sample system using appropriate positive and negative controls.
• For ELISA, consider direct antigen coating or capture formats. Titrate the antibody and validate with recombinant protein or cell lysates.
• Since E2F4 is phosphorylated, note that post-translational modifications may affect antibody recognition. Verify reactivity under your experimental conditions.
• The antibody shows cross-reactivity with human and mouse; ensure species compatibility when planning experiments.
• For cell cycle studies, synchronize cells to enrich for E2F4 expression during the G1/S transition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier provider of IVD raw materials, technical services, and consulting, supporting diagnostic manufacturers and research labs from concept to clinic. This anti-E2F4 antibody is part of our extensive portfolio of validated monoclonal and polyclonal antibodies for cell cycle and transcription research. Whether you need bulk quantities of high-affinity antibodies, optimized antibody pairs, or rare-target reagent sourcing, CamelBio's one-stop platform ensures reliable supply and expert support for your diagnostic development projects.