Anti-E2F2 Rabbit Monoclonal Antibody for WB and ELISA - Q14209

Core Product Specifications and Parameters

Biological Background: E2F2 Function and Localization

• Member of the E2F family of transcription factors, which play a crucial role in cell cycle regulation and DNA replication.
• Functions as a transcriptional activator by binding to the E2 recognition site (5'-TTTC[CG]CGC-3') in target promoters, cooperatively with DP proteins.
• The DRTF1/E2F complex, involving E2F2, controls the progression from G1 to S phase of the cell cycle.
• Specifically interacts with the retinoblastoma protein (RB1) in a cell-cycle-dependent manner.
• Predominantly localized in the nucleus.
• Post-translational modifications include phosphorylation (Phosphoprotein keyword).
• Highest expression is observed in placenta, with lower levels in lung; also detected in many immortalized tumor cell lines.
• The calculated molecular weight is approximately 48 kDa.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fusion protein spanning amino acids 303-395 of human E2F2. This C-terminal region may contribute to target-specific recognition.
• For Western blot, consider using positive control lysates from cells with high endogenous E2F2 expression (e.g., placental tissue or tumor cell lines). Validate specificity with appropriate knockdown or knockout controls.
• For ELISA applications, the antibody can serve as a capture or detection reagent. Optimal concentrations and assay conditions should be empirically determined.
• Cross-reactivity with mouse E2F2 is reported; verify performance in mouse samples if required for your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of monoclonal antibodies, including validated anti-E2F2 reagents for transcription and cell cycle research. We supply optimized antibodies, validated antibody pairs, and bulk ancillary raw materials to support diagnostic manufacturers and laboratories. For rare or application-specific targets, our team assists in sourcing custom raw materials, offering seamless support from concept to clinic.