Anti-E2F1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q01094

Core Product Specifications and Parameters

Biological Background: E2F1 Function and Localization

• E2F1 is a transcription factor that forms heterodimers with DP proteins to bind E2 recognition sites (5'-TTTC[CG]CGC-3') in target promoters. Related references: PMID:10675335, PMID:12717439, PMID:17050006
• The DRTF1/E2F complex regulates the G1/S transition by controlling expression of genes involved in cell cycle progression and DNA replication. Related references: PMID:10675335, PMID:12717439, PMID:17704056
• E2F1 interacts with the retinoblastoma protein RB1 in a cell-cycle-dependent manner, modulating its transcriptional activity. Related references: PMID:10675335, PMID:12717439, PMID:17704056
• Acts as a dual regulator, promoting both cell proliferation and TP53/p53-dependent apoptosis. Related references: PMID:8170954
• Directly activates transcription of PEG10 and RRP1B, and blocks adipocyte differentiation by repressing CEBPA binding to its target gene promoters. Related references: PMID:17050006, PMID:18625225, PMID:28992046, PMID:20040599, PMID:20176812
• Localizes primarily to the nucleus.
• Post-translationally modified by phosphorylation, acetylation, methylation, and ubiquitination, which regulate stability and activity.

Experimental Guidance and Technical Tips

• The predicted molecular weight of E2F1 is 47 kDa; for Western blotting, consider using an appropriate gel percentage and blocking conditions for this size range.
• For IF/ICC, effective nuclear permeabilization is critical; optimize fixation and permeabilization steps to ensure antibody access to the nuclear compartment.
• The immunogen corresponds to amino acids 200–301 of human E2F1; while cross-reactivity with mouse and rat is reported, validate detection specificity in your target species and tissue.
• This polyclonal antibody may recognize multiple E2F1 isoforms; confirm band patterns using appropriate controls and, if necessary, siRNA knockdown.
• For ELISA applications, consider checkerboard titration to determine optimal coating and detection antibody concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable one-stop partner for IVD raw material sourcing, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized development services. Our E2F1 antibody supports research in cell cycle regulation, apoptosis, and transcription factor biology, backed by a streamlined supply chain that accelerates your project from concept to clinic. Partner with us to access a wide portfolio of high-quality reagents and expert technical consultation.