Anti-E2A/TCF3 Rabbit Monoclonal Antibody for WB, ELISA - P15923

Core Product Specifications and Parameters

Biological Background: E2A/TCF3 Function and Localization

• E2A/TCF3 encodes two predominant isoforms, E12 and E47, generated by alternative splicing; both belong to the class B basic helix-loop-helix (bHLH) transcription factor family.
• It heterodimerizes with tissue-specific bHLH proteins to bind E-box motifs (5'-CANNTG-3') and specific enhancer elements, such as the kappa-E2 site in the immunoglobulin gene enhancer, thereby regulating cell fate in embryogenesis and B-cell differentiation (By similarity). Related references: PMID:2493990
• Together with TCF15, it is required for mesenchymal to epithelial transition (MET) (By similarity).
• It facilitates ATOH7 binding to DNA at the consensus sequence 5'-CAGGTG-3' and positively regulates its transcriptional activity.
• Subcellular localization is strictly nuclear, consistent with its role as a transcription factor.
• Post-translational modifications include phosphorylation, methylation, and ubiquitin-like conjugation; it is also classified as a proto-oncogene due to its involvement in chromosomal rearrangements.

Experimental Guidance and Technical Tips

• For Western blot analysis, a band around 67-68 kDa is expected; ensure appropriate gel percentage and validate detection in relevant human or mouse cell lysates (e.g., B-cell lines) given the cross-reactivity with human and mouse.
• For ELISA applications, consider optimizing the antibody coating concentration and antigen source; pairing with a matched detection antibody may improve assay sensitivity.
• The immunogen corresponds to amino acids 379-525, which lies within the bHLH domain; note that alternative splicing isoforms may affect antibody recognition in some assays.

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