Anti-E-Selectin/CD62E Polyclonal Antibody for WB, ELISA - Q00690

Core Product Specifications and Parameters

Biological Background: E-Selectin/CD62E Function and Localization

• E-selectin (CD62E, ELAM-1, LECAM2) is a cell surface glycoprotein encoded by the Sele gene in mice. It belongs to the selectin family of adhesion molecules and functions primarily in immune surveillance and inflammation.
• Core function: Mediates the initial adhesion and rolling of blood neutrophils on cytokine-activated endothelial cells by interacting with its ligand PSGL-1 (SELPLG). Related references: PMID:1375914
• May also contribute to capillary morphogenesis, suggesting a role in angiogenesis and new blood vessel formation.
• Predominantly localized to the cell membrane as a type I transmembrane protein; its extracellular region can be shed upon activation.
• Structural domains include an N-terminal C-type lectin domain, an EGF-like domain, multiple Sushi (SCR/CCP) repeats, a single transmembrane helix, and a short cytoplasmic tail. Calcium binding to the lectin domain is essential for ligand recognition.
• Contains multiple disulfide bonds that stabilize the extracellular domains and is extensively glycosylated.
• Along with L-selectin and P-selectin, it forms the selectin family, each exhibiting distinct but overlapping patterns of expression and ligand specificity.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a recombinant protein spanning the extracellular domain (aa 22-557) of mouse E-selectin; it is expected to recognize both the full-length membrane-bound form and the soluble shed ectodomain.
• For Western blot, a band at approximately 66 kDa (predicted molecular weight ~66.7 kDa) should be observed; be aware that glycosylation may cause slight migration shifts. Titration of both primary and secondary antibodies is recommended.
• In ELISA applications, the antibody can be used for capture or detection of recombinant or endogenous E-selectin. Validate with appropriate positive and negative controls in your assay system.
• The product is validated for reactivity with mouse samples; cross-reactivity with other species has not been determined.
• Because the immunogen covers most of the extracellular region, it may be suitable for detecting denatured protein under reducing conditions; however, native conformation-dependent applications may require empirical optimization.
• Consider using a 5% non-fat milk or BSA blocking solution to reduce non-specific binding, especially for Western blot of tissue lysates.

CamelBio: Your One-Stop Sourcing Bridge

This E-Selectin/CD62E antibody supports research into leukocyte adhesion, inflammation, and endothelial biology—critical areas for IVD development. CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and flexible sourcing for rare targets, providing a single point of contact for diagnostic raw-material procurement from discovery to scale-up.