Anti-E-Cadherin/CD324 Monoclonal Antibody for Flow Cytometry - P12830

Core Product Specifications and Parameters

Biological Background: E-Cadherin Function and Localization

• Cadherin-1 (E-Cadherin, Epithelial cadherin, Uvomorulin, CD324) is a calcium-dependent cell adhesion glycoprotein that mediates homophilic interactions, contributing to epithelial cell sorting and tissue integrity. Related references: PMID:11976333
• E-Cadherin plays a critical role in regulating epithelial cell mobility, proliferation, and acts as a potent invasion suppressor; it also functions as a ligand for integrin αEβ7. Related references: PMID:11976333
• It facilitates early desmosome assembly by recruiting desmoglein-2 (DSG2) and desmoplakin (DSP) to nascent cell-cell junctions. Related references: PMID:29999492
• The protein organizes radial actin fibers at adherens junctions via interaction with AMOTL2, mediating cellular responses to contractile forces (By similarity).
• Subcellularly, E-Cadherin localizes to the cell membrane at adherens junctions and desmosomes, and is also found in the Golgi, endosomes, and as a secreted ectodomain.
• Tissue expression includes basal epithelial layers of skin and esophagus, liver, and granuloma macrophages.
• Post-translational modifications include phosphorylation, glycosylation, disulfide bond formation, and proteolytic cleavage generating fragments such as E-Cad/CTF2 that modulate APP processing.
• (Microbial infection) E-Cadherin serves as a receptor for Listeria monocytogenes internalin A, facilitating bacterial entry.

Experimental Guidance and Technical Tips

• This antibody targets the extracellular domain (aa 155-707) of human E-Cadherin; it is suitable for flow cytometry detection of surface expression.
• Titrate and optimize staining conditions for the specific cell type; consider using isotype controls and Fc receptor blocking to reduce background.
• For intracellular staining, ensure that fixation and permeabilization protocols are compatible with antigen recognition.
• The antibody is supplied as an conjugate; verify laser and filter compatibility (e.g., 405 nm excitation, 450/50 nm emission).
• Validate gating strategies and, if needed, confirm expression with an orthogonal method, given the ~97 kDa molecular weight.
• Reactivity has been validated in human; cross-reactivity with other species has not been determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing solution for IVD raw materials, supporting diagnostic manufacturers and researchers with validated antibodies, recombinant proteins, and ancillary reagents. This anti-E-Cadherin monoclonal antibody is part of our cell adhesion and oncology portfolio, enabling flow cytometry applications. Our team specializes in supplying optimized monoclonal antibodies, bulk reagents, and custom sourcing for rare targets to accelerate your diagnostic development.