Anti-E-Cadherin Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - P12830

Core Product Specifications and Parameters

Biological Background: E-Cadherin Function and Localization

• Cadherin-1 (E-cadherin) is a calcium-dependent cell adhesion protein that mediates homophilic interactions to connect cells, contributing to the sorting of heterogeneous cell types (PubMed:11976333).
  Related references: PMID:11976333

• E-cadherin is involved in regulating cell-cell adhesions, mobility, and proliferation of epithelial cells (PubMed:11976333).
  Related references: PMID:11976333

• It plays a role in the early stages of desmosome formation by facilitating the recruitment of DSG2 and DSP to desmosome plaques (PubMed:29999492).
  Related references: PMID:29999492

• Functions as a potent invasive suppressor and serves as a ligand for integrin alpha-E/beta-7.

• The cytoplasmic tail fragment E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors, inhibiting APP C99 and C83 production.

• Acts as a receptor for Listeria monocytogenes internalin A, promoting bacterial uptake.

• Subcellularly localized to adherens junctions, desmosomes, cell membrane, endosome, Golgi, and can be secreted.

• Expressed in epithelial tissues including skin, esophageal epithelium, and liver; also found in granuloma macrophages.

Experimental Guidance and Technical Tips

• For Western blot (WB), under denaturing conditions, a band around 97 kDa is expected. Include appropriate loading controls and validate in your sample system.
• For immunohistochemistry (IHC-P), optimize antigen retrieval as E-cadherin is a transmembrane protein. Test on skin or esophageal epithelium as positive control tissues.
• The immunogen is a synthetic peptide from the cytoplasmic domain (aa 701-800); it may detect both full-length and cleaved fragments. Consider this when analyzing bands of different sizes.
• For immunofluorescence (IF), perform fixation and permeabilization steps carefully. Co-staining with junctional markers may confirm proper localization.
• Cross-reactivity with mouse and rat is indicated; perform appropriate species-specific controls when using these models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, dedicated to supporting diagnostic manufacturers, laboratories, and research institutes from concept to clinic. This anti-E-Cadherin monoclonal antibody is ideal for studies in cell adhesion, epithelial-mesenchymal transition, and cancer biology. We offer a comprehensive portfolio including validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, ensuring all your E-Cadherin research and development needs are met with efficiency and quality.