Anti-E-Cadherin Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P12830

Core Product Specifications and Parameters

Biological Background: E-Cadherin Function and Localization

• E-Cadherin (Cadherin-1, also known as CAM 120/80, Epithelial cadherin, Uvomorulin) is a classical cadherin encoded by the CDH1 gene. It belongs to the cadherin superfamily of calcium-dependent cell adhesion molecules.
• It mediates homophilic cell-cell adhesion at adherens junctions, which is essential for maintaining epithelial tissue integrity and architecture. Related references: PMID:11976333
• E-Cadherin regulates cell sorting, mobility, and proliferation. Through interaction with AMOTL2, it anchors radial actin fibers to junctional complexes and transduces contractile forces.
• It facilitates early desmosome assembly by recruiting desmoglein-2 (DSG2) and desmoplakin (DSP) to desmosomal plaques. Related references: PMID:29999492
• The protein localizes to the cell membrane, adherens junctions, and desmosomes; it also cycles through the endosomal–Golgi network and can be secreted.
• Post-translational modifications include cleavage, phosphorylation, ubiquitination, disulfide bond formation, and N‑glycosylation.
• Expressed in epithelial tissues (skin, liver, esophageal epithelium) and in granuloma macrophages. Related references: PMID:27760340 PMID:22294297 PMID:24220297 PMID:3263290
• Serves as a receptor for Listeria monocytogenes internalin A, promoting bacterial entry into host cells.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 373–622, which lies within the extracellular cadherin repeat region; consider that the antibody may recognize both full-length E-Cadherin and proteolytic fragments.
• Predicted molecular weight is ~97 kDa; however, due to extensive glycosylation and propeptide processing, the mature protein typically migrates at 120–135 kDa in SDS-PAGE. Validate in your sample system.
• For IHC-P, perform heat-induced antigen retrieval in citrate buffer (pH 6.0) and optimize primary antibody dilution.
• Cross-reactivity is expected with mouse and rat based on sequence homology; verify for your specific application.
• Titrate the antibody for ELISA and IF/ICC to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinical application. For E-Cadherin-targeted assays, we can supply validated antibody pairs, bulk monoclonal/polyclonal reagents, and ancillary assay components. Our sourcing platform also handles custom requirements for rare or challenging targets, providing a seamless procurement experience.