Anti-E-Cadherin Polyclonal Antibody for WB, IHC-P, ELISA - P12830

Core Product Specifications and Parameters

Biological Background: E-Cadherin Function and Localization

• E-Cadherin (CDH1) is a calcium-dependent cell adhesion protein that mediates homophilic interactions essential for tissue integrity and cell sorting. It also plays a role in regulating epithelial cell mobility and proliferation. Related references: PMID:11976333

• It is a key component of adherens junctions and contributes to the early formation of desmosomes by facilitating recruitment of DSG2 and DSP. Related references: PMID:29999492

• The protein possesses a potent invasive suppressor function and acts as a ligand for integrin alpha-E/beta-7.

• E-Cadherin also promotes non-amyloidogenic degradation of Abeta precursors and inhibits APP C99 and C83 production.

• It serves as a receptor for Listeria monocytogenes internalin A, mediating bacterial uptake.

• Subcellularly, it localizes to the cell membrane, adherens junctions, desmosomes, and can also be found in the Golgi, endosomes, and as a secreted fragment following proteolytic cleavage.

• Tissue expression is prominent in the epithelial layers of skin, esophagus, and liver, as well as in granuloma macrophages.

• Post-translational modifications include phosphorylation, glycosylation, disulfide bond formation, and ubiquitination, which regulate its stability and function.

Experimental Guidance and Technical Tips

• The immunogen (aa 373–622) lies within the extracellular domain of E-Cadherin, making this antibody suitable for detecting the full-length transmembrane protein in western blot (WB) at ~97 kDa.
• For immunohistochemistry on paraffin-embedded sections (IHC-P), antigen retrieval may be necessary; validate with known positive and negative control tissues.
• ELISA performance can be optimized using recombinant E-Cadherin protein; consider pairing with a detection antibody for quantitative assays.
• Predicted cross-reactivity with mouse and rat E-Cadherin, but always confirm specificity in the relevant experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive procurement partner for IVD manufacturers and research laboratories, offering validated antibody pairs, recombinant proteins, and ancillary reagents to support E-Cadherin-based diagnostic assay development. Whether you need bulk quantities of this polyclonal antibody, matched detection reagents, or assistance sourcing rare targets, our one-stop platform streamlines your raw-material supply chain—from initial feasibility to clinical application.