Anti-E-Cadherin Monoclonal Antibody for IHC-P, ELISA - P09803

Core Product Specifications and Parameters

Biological Background: Cadherin-1 Function and Localization

• Cadherin-1 (also known as E-cadherin, Uvomorulin, ARC-1) is a calcium-dependent cell adhesion protein that mediates homophilic cell-cell interactions, contributing to the sorting of heterogeneous cell types and regulating epithelial cell adhesion, mobility, and proliferation. Related references: PMID:11976333
• It promotes the organization of radial actin fiber structure and cellular response to contractile forces by anchoring radial actin fibers to junction complexes at the cell membrane via interaction with AMOTL2.
• CDH1 plays a role in the early stages of desmosome cell-cell junction formation by facilitating the recruitment of DSG2 and DSP to desmosome plaques.
• It acts as a potent invasion suppressor and serves as a ligand for integrin alpha-E/beta-7. Additionally, the E-Cad/CTF2 fragment promotes non-amyloidogenic degradation of Abeta precursors and inhibits APP C99 and C83 production.
• In mice, CDH1 is expressed in non-neural epithelial tissues, with specific detection in inner and outer pillar cells of the organ of Corti, granuloma macrophages, and epidermal keratinocytes from birth onward. Related references: PMID:30639848, PMID:27760340, PMID:18079750, PMID:27375112, PMID:30660666
• It localizes to the cell membrane, adherens junctions, desmosomes, endosomes, and the Golgi apparatus trans-Golgi network, with cytoplasmic localization also observed.
• Post-translational modifications include signal peptide cleavage, disulfide bond formation, phosphorylation, and ubiquitin conjugation, consistent with its dynamic role in cell adhesion and signaling.
• Notably, this mouse cadherin does not function as a receptor for Listeria monocytogenes internalin A without a specific surface residue mutation.

Experimental Guidance and Technical Tips

• The antibody is generated against recombinant mouse E-Cadherin protein; verify specificity in your experimental system using appropriate positive and negative controls.
• For IHC-P, consider optimizing antigen retrieval and antibody dilution to achieve clear membrane staining in epithelial tissues from human, mouse, or rat samples.
• In ELISA, use as a capture or detection antibody after confirming linearity and sensitivity with recombinant protein or cell lysates.
• Given its cross-reactivity with human, mouse, and rat, this antibody is suitable for comparative studies across species; however, validate expression levels in each species separately.
• Because E-Cadherin undergoes proteolytic processing, be aware of potential band shifts if exploring other applications; the full-length protein is ~98kDa.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For E-Cadherin research, we can source validated antibody pairs, optimized monoclonal antibodies like this product, and bulk ancillary reagents to support your epithelial biology and cell adhesion studies. Our global supply network enables efficient procurement of rare-target raw materials, accelerating your assay development and commercialization timelines.