Anti-Dystrophin Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P11532

Core Product Specifications and Parameters

Biological Background: Dystrophin Function and Localization

• Dystrophin, encoded by the DMD gene, is a large cytoskeletal protein produced as multiple isoforms through alternative splicing and alternative promoter usage.
• It serves as a core component of the dystrophin-associated glycoprotein complex (DGC), anchoring the extracellular matrix to the intracellular F-actin cytoskeleton by binding dystroglycan; this complex is critical for stabilizing the sarcolemma during muscle contraction.
• The DGC concentrates at the neuromuscular junction (NMJ) and at diverse synapses in the central and peripheral nervous systems, where dystrophin supports structural integrity and synaptic transmission.
• Dystrophin is predominantly located in muscle fibers at costameres of the sarcolemma; it is also expressed in brain, kidney, lung, and testis, with isoform 15 uniquely found in heart and liver.
• Subcellularly, dystrophin is present at the cell membrane, sarcolemma, postsynaptic cell membrane, and associated with the cytoskeleton, consistent with its roles in membrane stability and signal transduction.
• The protein encompasses actin-binding regions, spectrin-like repeats, zinc-finger motifs, and calcium-binding domains, undergoes phosphorylation, and mutations in DMD lead to Duchenne/Becker muscular dystrophy and cardiomyopathy.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a segment of dystrophin (amino acids 346–635), representing a conserved mid‑region of the protein. Validate the antibody’s reactivity with isoforms expressed in your sample systems.
• For Western blotting, consider that dystrophin is exceptionally large (~427 kDa). Extended transfer times and appropriate gel systems are often necessary to achieve efficient membrane transfer.
• Perform standard validation in relevant positive controls (e.g., human, mouse, or rat skeletal muscle lysates) and negative controls to confirm specificity across applications (WB, IF-P, IHC-P, ELISA).
• In immunofluorescence‑based protocols, ensure permeabilization steps are optimized for detection of this predominantly membrane‑associated protein.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high‑quality IVD raw materials for neuromuscular and muscular dystrophy targets. As a one‑stop sourcing partner, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and bespoke supply of rare‑target raw materials—empowering your assay development from concept to clinic.