Anti-DYRK4 Rabbit Polyclonal Antibody for WB, ELISA - Q9NR20

Core Product Specifications and Parameters

Biological Background: DYRK4 Function and Localization

• Dual specificity tyrosine-phosphorylation-regulated kinase 4 (DYRK4) is a serine/threonine and tyrosine protein kinase that belongs to the DYRK family of kinases.
• DYRK4 may play a non-essential role in spermiogenesis, the final stage of sperm cell development.
• The protein is found in both the cytoplasm and the nucleus, suggesting nucleocytoplasmic shuttling or dual functions.
• DYRK4 gene undergoes alternative splicing, producing multiple transcript variants with potentially distinct roles.
• The kinase requires magnesium ions for catalytic activity and is regulated by phosphorylation on tyrosine and serine/threonine residues.
• DYRK4 has been identified in high-throughput proteomics studies, confirming its expression in human tissues.
• No disease association or critical phenotype has been definitively linked to DYRK4 loss of function, consistent with its possible non-essential role.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 330-480 of human DYRK4; for endogenous protein detection, verify antibody binding in the relevant tissue or cell line due to possible post-translational modifications or protein interactions.
• For Western blot, consider using a denaturing gel and appropriate molecular weight marker; predicted molecular weight is approximately 60 kDa, but actual migration may vary.
• In ELISA, coating conditions and antibody dilutions should be optimized empirically using recombinant DYRK4 or positive control lysates.
• Cross-reactivity with mouse and rat DYRK4 is indicated; validate specificity in these species using knockout controls or peptide competition if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines access to IVD raw materials for diagnostic developers and research laboratories, from early-stage discovery to clinical readiness. Whether you require validated antibody pairs, optimized monoclonal/polyclonal antibodies like our anti-DYRK4 pAb, or bulk ancillary reagents, we support your workflow with reliable sourcing and technical consultation. For targets such as DYRK4 involved in spermiogenesis, we can help bridge gaps in rare or pathway-specific reagent supplies to keep your development on track.