Anti-DYRK2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q92630

Core Product Specifications and Parameters

Biological Background: DYRK2 Function and Localization

• DYRK2 is a dual-specificity serine/threonine and tyrosine kinase involved in regulating the mitotic cell cycle, apoptosis, cytoskeleton organization, and neurite outgrowth. Related references: PMID:11311121 PMID:12588975 PMID:16511445

• Functions downstream of ATM in the DNA damage response by phosphorylating p53/TP53 at Ser-46, thereby promoting apoptosis. Related references: PMID:17349958 PMID:18599021

• Serves as a priming kinase for GSK3 substrates such as EIF2B5, NFATC1, CRMP2/DPYSL2, and CRMP4/DPYSL3, facilitating their subsequent phosphorylation by GSK3B. Related references: PMID:11311121 PMID:16611631

• Promotes ubiquitin-dependent proteasomal degradation of multiple targets including KATNA1, TERT, MYC, and JUN, playing a critical role in cell cycle progression and proliferation control. Related references: PMID:19287380 PMID:23362280 PMID:22307329

• Can phosphorylate histone H1, H2B, and H3 in vitro, and also phosphorylates CARHSP1 in vitro. Related references: PMID:9748265 PMID:15910284

• Acts as a dual regulator of smoothened signaling by phosphorylating GLI2 and GLI3, either promoting their dissociation from SUFU and nuclear translocation, or triggering their proteasomal degradation. Related references: PMID:38968120 PMID:18455992

• Localizes to both the cytoplasm and nucleus, and its expression is noted in testis after the onset of spermatogenesis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 500–600 of human DYRK2; this region shares high sequence identity with rat DYRK2, supporting the observed cross-reactivity.
• For Western blot (WB), consider using whole-cell lysates from human or rat cell lines; DYRK2 typically runs at 66 kDa but may appear as a doublet around 59–66 kDa due to isoforms or post-translational modifications.
• In immunohistochemistry (IHC-P) on paraffin-embedded tissues, validate in testis or tumor samples where DYRK2 expression is expected; antigen retrieval may be required.
• For ELISA, coat plates with the immunogen peptide or use a sandwich format with a capture/detection antibody pair.
• Because DYRK2 is a kinase with multiple phosphorylation substrates, inclusion of phosphatase inhibitors in lysis buffers can help preserve phospho-epitope integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive sourcing solutions for IVD raw materials, supporting diagnostic manufacturers and research laboratories from initial concept through clinical application. This anti-DYRK2 rabbit polyclonal antibody is part of our extensive catalog of validated antibodies tailored for research in cell cycle regulation, apoptosis, and cancer biology. In addition to individual antibodies, CamelBio offers optimized monoclonal/polyclonal antibody development, bulk ancillary reagents, and rare-target raw-material procurement to streamline your assay development pipeline.