Anti-DYRK2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q92630

Core Product Specifications and Parameters

Biological Background: DYRK2 Function and Localization

• DYRK2 is a dual-specificity serine/threonine- and tyrosine-protein kinase involved in the regulation of the mitotic cell cycle, cell proliferation, apoptosis, cytoskeleton organization, and neurite outgrowth. Related references: PMID:11311121, PMID:12588975, PMID:16511445

• It phosphorylates a broad range of substrates, including CRMP2/DPYSL2, CRMP4/DPYSL3, DCX, EIF2B5, EIF4EBP1, GLI2, GLI3, GYS1, JUN, MDM2, MYC, NFATC1, p53/TP53, TAU/MAPT, KATNA1, and histones H1, H2B, and H3. Related references: PMID:11311121, PMID:12588975, PMID:16511445, PMID:9748265, PMID:15910284

• DYRK2 serves as a priming kinase for GSK3 substrates: it phosphorylates EIF2B5 at Ser-544, enabling subsequent inhibition by GSK3B, and similarly primes NFATC1, CRMP2, and CRMP4 for GSK3B-dependent phosphorylation. Related references: PMID:11311121, PMID:16611631

• Downstream of ATM, DYRK2 phosphorylates p53 at Ser-46, contributing to DNA damage-induced apoptosis. Related references: PMID:17349958, PMID:18599021

• The kinase inactivates glycogen synthase 1 (GYS1) by phosphorylation at Ser-641, thereby regulating glycogen synthesis. Related references: PMID:14593110

• DYRK2 promotes ubiquitin-dependent proteasomal degradation: it mediates EDVP E3 ligase complex assembly for KATNA1 degradation, phosphorylates TERT to trigger its ubiquitination, and targets MYC and JUN for degradation to control cell proliferation. Related references: PMID:19287380, PMID:23362280, PMID:22307329

• In Hedgehog signaling, DYRK2 phosphorylates GLI2 and GLI3, facilitating their dissociation from SUFU and nuclear translocation, while also promoting their degradation in a context-dependent manner. Related references: PMID:38968120, PMID:18455992

• DYRK2 is predominantly expressed in the testis after the onset of spermatogenesis, and the protein localizes to both the nucleus and cytoplasm. Multiple isoforms are generated through alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 429-528 of human DYRK2 (NP_003574.1), a region near the C-terminus. This sequence is conserved in mouse DYRK2, supporting the reported cross-reactivity with mouse samples. Researchers may consider sequence alignment to assess reactivity in other species.
• For Western blot analysis, a band of approximately 67 kDa corresponding to full-length DYRK2 is expected. Additional bands may appear due to alternative splicing; validate in the relevant sample system.
• In immunofluorescence/immunocytochemistry (IF/ICC), both nuclear and cytoplasmic staining are anticipated, reflecting endogenous DYRK2 localization. Appropriate fixation and permeabilization protocols should be optimized.
• This antibody can be employed in ELISA-based assays. Consider pairing with a suitable detection antibody and validating in the target matrix to ensure specificity.
• Given the potential for cross-reactivity with other DYRK family members, it is recommended to verify specificity using knockdown or knockout controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop sourcing solution for IVD raw materials, supporting every stage from concept to clinic. This anti-DYRK2 polyclonal antibody is part of CamelBio's extensive catalog of kinase and cell signaling research reagents. In addition to validated antibodies, we supply optimized antibody pairs, bulk ancillary reagents, and can source rare-target raw materials to streamline your assay development and production workflows.