Anti-DYRK1A Polyclonal Antibody for WB, ELISA - Q13627

Core Product Specifications and Parameters

Biological Background: DYRK1A Function and Localization

• Encodes dual specificity tyrosine-phosphorylation-regulated kinase 1A, also known as DYRK, MNB, MNBH, and HP86; a member of the DYRK family of dual-specificity kinases.
• Functions as a dual-specificity kinase with both serine/threonine and tyrosine kinase activities, preferentially phosphorylating substrates with proline at position P+1 and arginine at P-3. Related references: PMID:20981014 PMID:21127067 PMID:23665168
• Plays a critical role in DNA double-strand break repair; phosphorylates RNF169 to enhance its ability to block TP53BP1 accumulation at damage sites, thereby promoting homologous recombination. Related references: PMID:30773093 PMID:31024071
• Acts as a positive transcription regulator by phosphorylating the C-terminal domain of RNA polymerase II, and modulates alternative splicing through phosphorylation of the splice factor SRSF6. Related references: PMID:25620562 PMID:29849146
• Involved in a signaling pathway regulating nuclear functions of cell proliferation; also exhibits pro-survival activity by negatively regulating apoptosis. Related references: PMID:14500717
• Localizes predominantly in the nucleus and is enriched in nuclear speckles.
• Ubiquitously expressed with highest levels in skeletal muscle, testis, fetal lung, and fetal kidney.
• Associated with intellectual disability (MRD7) and host-virus interactions; phosphoprotein regulated by autophosphorylation and other modifications.

Experimental Guidance and Technical Tips

• For Western blotting, a band corresponding to approximately 86 kDa is expected; ensure use of an appropriate gel percentage for high molecular weight proteins.
• ELISA may be used for quantification of DYRK1A levels; consider using the recombinant immunogen or a recombinant protein standard for calibration.
• As a rabbit polyclonal antibody, batch-to-batch consistency should be verified; validate performance in the relevant sample system and consider titration for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with one-stop access to validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents for targets like DYRK1A. Our sourcing capabilities extend to rare and specialized kinases, recombinant proteins, and custom development from concept to clinic. Whether you need critical raw materials for DNA repair or transcription-related IVD assays, CamelBio streamlines your procurement with reliable supply chains.