Anti-DYNLL2 Polyclonal Antibody for WB and ELISA - Q96FJ2

Core Product Specifications and Parameters

Biological Background: DYNLL2 Function and Localization

• DYNLL2 (Dynein light chain 2, cytoplasmic) is a 10 kDa protein also known as 8 kDa dynein light chain b (DLC8b) or dynein light chain LC8-type 2.
• It functions as a non-catalytic accessory subunit of the cytoplasmic dynein 1 complex, facilitating the linking of dynein to cargoes and adapter proteins.
• Cytoplasmic dynein 1 is a microtubule motor responsible for intracellular retrograde transport of vesicles and organelles.
• DYNLL2 may contribute to the regulation of cytoskeletal structure distribution within the cell.
• It localizes to the cytoplasm and associates with the cytoskeleton, consistent with its role in dynein-mediated motility.
• The protein is evolutionarily conserved and belongs to the dynein light chain family, characterized by its small size and dynein-binding function.
• Keywords from UniProt include dynein, microtubule, motor protein, and transport, highlighting its central role in cellular logistics.

Experimental Guidance and Technical Tips

• For Western blot (WB) applications, expect a specific band at approximately 10 kDa corresponding to DYNLL2 in cell or tissue lysates from human, mouse, or rat sources. Validate with positive and negative controls.
• In ELISA, this antibody may be suitable for detecting recombinant or endogenous DYNLL2; standardize coating and detection conditions.
• Cross-reactivity with mouse and rat DYNLL2 has been noted; verify consistency in your model system, as sequence homology may affect binding.
• The immunogen spans amino acids 1-89, the full-length protein, suggesting potential binding to both native and denatured forms. Nevertheless, affinity may vary; consider optimizing incubation and blocking steps.
• As a polyclonal antibody, lot-to-lot variability can occur. Always include internal reference samples to monitor performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable IVD raw-material sourcing, from validated antibody pairs to bulk ancillary reagents. This anti-DYNLL2 polyclonal antibody supports research in intracellular transport and dynein biology applications. For projects requiring rare target reagents or custom solutions, our team streamlines procurement to accelerate your development pipelines.