Anti-DYNLL1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P63167

Core Product Specifications and Parameters

Biological Background: DYNLL1 Function and Localization

• Component of cytoplasmic dynein 1, a motor protein complex that drives retrograde transport of vesicles and organelles along microtubules.
• Acts as a non-catalytic accessory subunit linking dynein to cargos and adapter proteins, thereby regulating dynein motor function.
• Functions as a protein-protein adapter that inhibits and/or sequesters target proteins, including neuronal nitric oxide synthase (NOS1) and BCL2L11. Related references: PMID:10198631 PMID:15193260 PMID:15891768
• Key regulator of the DNA damage response: upon phosphorylation at Ser-88, recruited by TP53BP1 to DNA double-strand breaks (DSBs) to disrupt MRE11 dimerization and inhibit DNA end resection. In a subset of DSBs, unphosphorylated DYNLL1 promotes recruitment of the Shieldin complex. Related references: PMID:30464262 PMID:37696958
• Mediates apoptosis by sequestering BCL2L11 to microtubules; upon apoptotic stimuli, the BCL2L11-DYNLL1 complex dissociates and translocates to mitochondria, where it sequesters BCL2 and neutralizes its anti-apoptotic activity. Related references: PMID:10198631 PMID:15193260
• Enhances estrogen receptor alpha (ESR1) transactivation and helps direct its nuclear localization. Related references: PMID:15891768 PMID:16684779
• Ubiquitously expressed in tissues, with prominent expression detected in testis.
• Subcellular distribution includes the cytoplasm, cytoskeleton (centrosome, microtubules), nucleus, chromosomes, and mitochondria.
• Post-translational modifications include phosphorylation and acetylation, regulating its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-89 of human DYNLL1, targeting an N-terminal region. Consider this when designing epitope mapping or competition assays.
• For western blotting (WB), the predicted molecular weight is approximately 10 kDa. Use high-percentage SDS-PAGE gels and suitable membrane pore size for efficient transfer of low-molecular-weight proteins.
• For immunohistochemistry (IHC-P), antigen retrieval conditions may require optimization depending on tissue type and fixation method.
• ELISA protocols may benefit from cross-reactivity testing against the synthetic peptide immunogen as a positive control.
• Validate antibody specificity in relevant biological systems, such as DYNLL1 knockout or knockdown cells, to confirm target recognition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions streamlined access to a wide range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk reagents, and ancillary supplies. This anti-DYNLL1 monoclonal antibody serves cytoskeleton, apoptosis, and DNA damage response research, and we can source complementary rare-target reagents to accelerate your assay development. Partner with CamelBio to bridge the gap from concept to clinic with reliable, one-stop procurement support.