Anti-DYNLL1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P63167

Core Product Specifications and Parameters

Biological Background: DYNLL1 Function and Localization

• DYNLL1 is a component of cytoplasmic dynein 1, acting as a non-catalytic accessory subunit that links the motor complex to cargo adapters and regulators, facilitating microtubule-based retrograde transport of vesicles and organelles.
• Functions as a protein-protein adapter that binds and inhibits multiple target proteins, including neuronal nitric oxide synthase (NOS1) and the pro-apoptotic factor BCL2L11, thereby modulating diverse signaling pathways. Related references: PMID:10198631, PMID:15193260, PMID:15891768
• Plays a critical role in apoptosis regulation by sequestering BCL2L11 to microtubules; upon apoptotic stimuli, the DYNLL1-BCL2L11 complex dissociates from dynein, translocates to mitochondria, and neutralizes BCL2's anti-apoptotic activity. Related references: PMID:10198631, PMID:15193260
• Involved in the DNA damage response: when phosphorylated at Ser-88, DYNLL1 is recruited by TP53BP1 to DNA double-strand breaks, where it disrupts MRE11 dimerization to inhibit DNA end resection; unphosphorylated DYNLL1 promotes recruitment of the Shieldin complex at a subset of breaks. Related references: PMID:30464262, PMID:37696958
• Promotes the transactivation functions and nuclear localization of estrogen receptor alpha (ESR1), linking dynein to hormone signaling. Related references: PMID:15891768, PMID:16684779
• Localizes to multiple subcellular compartments, including cytoplasm, cytoskeleton (microtubules and centrosomes), chromosomes, nucleus, and mitochondria, reflecting its versatile adapter roles.
• Ubiquitously expressed, with notable expression in testis, consistent with its fundamental role in intracellular transport and signaling. Related references: PMID:8628263, PMID:22965910

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-89 amino acids of human DYNLL1, which is highly conserved across human, mouse, and rat, supporting cross-reactivity. Validate antibody specificity in the intended species and sample type.
• For western blot, DYNLL1 migrates at approximately 10 kDa; use a high-percentage SDS-PAGE gel (e.g., 15%) and ensure efficient transfer for low molecular weight proteins.
• In immunoprecipitation and ELISA applications, consider that DYNLL1 functions as a homodimer; native conformation may affect antibody binding.
• For apoptosis studies, monitor subcellular relocalization from microtubules to mitochondria post-stimulus; include appropriate positive controls (e.g., staurosporine-treated cells).
• In DNA damage response experiments, detection of phosphorylated Ser-88 may require specific induction conditions (e.g., ionizing radiation) and phosphatase inhibitors.

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