Anti-Dynein intermediate chain 1 Rabbit Monoclonal Antibody for WB, ELISA - Q9UI46

Core Product Specifications and Parameters

Biological Background: Dynein intermediate chain 1 Function and Localization

• The DNAI1 gene encodes dynein axonemal intermediate chain 1, a WD repeat-containing protein that serves as a critical structural and functional component of the outer dynein arm complex in motile cilia and flagella.
• This protein is essential for ciliary movement along microtubules, generating the force required for mucociliary clearance in the respiratory tract and for sperm motility.
• Multiple protein isoforms are produced through alternative splicing, which may contribute to tissue-specific regulation of dynein assembly and function in different ciliated cell types.
• At the protein level, DNAI1 is predominantly expressed in respiratory ciliated epithelial cells, aligning with its role in protecting the airways from pathogen and particulate accumulation.
• Subcellularly, DNAI1 localizes along the entire axoneme of cilia and flagella, anchored to the outer doublet microtubules as part of the dynein motor complex.
• Mutations in DNAI1 are associated with primary ciliary dyskinesia (PCD) and Kartagener syndrome, disorders characterized by chronic respiratory infections, situs inversus, and male infertility due to immotile cilia and sperm.
• Post‑translational modifications, particularly phosphorylation, regulate dynein activity and axonemal dynamics, though the precise modification sites on DNAI1 remain to be fully mapped.
• The N‑terminal region contains WD40 repeat domains that mediate protein‑protein interactions essential for dynein complex integrity and motor force generation.

Experimental Guidance and Technical Tips

• For Western blot detection, prepare lysates from ciliated epithelial cell lines or tissues to confirm endogenous expression; a band around 79 kDa is expected.
• Use standard SDS‑PAGE conditions (e.g., 8–10% gel) and optimize transfer for high‑molecular‑weight proteins to ensure efficient detection.
• Since the immunogen spans the N‑terminal 1–200 amino acids, the antibody may recognize multiple isoforms; validate specificity using knockdown or knockout controls if available.
• For ELISA applications, coat the plate with the recombinant immunogen or purified protein and titrate the antibody to establish a suitable working concentration.
• As cross‑reactivity with mouse and rat is reported, this antibody can be considered for studies in these species, but proper species‑specific protocol optimization is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated provider of IVD raw materials, offering reliable access to high‑quality monoclonal antibodies such as this anti‑DNAI1 clone, which supports research into ciliopathies and respiratory disease. Beyond individual antibodies, we supply validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, accelerating your diagnostic pipeline from concept to clinical application. Partner with CamelBio to streamline your procurement and technical challenges in molecular diagnostics.