Anti-DYNC2LI1 Rabbit Polyclonal Antibody for WB, ELISA - Q8TCX1

Core Product Specifications and Parameters

Biological Background: DYNC2LI1 Function and Localization

• DYNC2LI1 encodes the cytoplasmic dynein 2 light intermediate chain 1, a non-catalytic accessory subunit of the retrograde motor dynein-2 complex essential for intraflagellar transport and ciliary assembly. Related references: PMID:29742051
• The protein plays a critical role in regulating ciliary length, impacting proper cilia formation and signaling. Related references: PMID:26077881, PMID:26130459
• DYNC2LI1 is part of the dynein heavy chain family; alternative splicing produces multiple isoforms, potentially diversifying its function.
• Subcellularly, DYNC2LI1 localizes to the Golgi apparatus, cytoplasm, ciliary axoneme, basal body, and centrosome, consistent with its role in ciliary trafficking.
• Expressed predominantly in bone, brain, kidney, and cartilage, with lower levels in heart, liver, lung, placenta, and thymus.
• Keywords highlight its involvement in ciliopathies and disease variants, underscoring its medical relevance in ciliary disorders.
• The protein has a molecular weight of approximately 40 kDa (calculated 39.6 kDa) and is amenable to detection by standard immunochemical methods.

Experimental Guidance and Technical Tips

• Since the immunogen corresponds to the N-terminal 352 amino acids, the antibody likely recognizes multiple isoforms; validate in ciliated cell lines or tissues.
• For Western blotting, consider optimizing antibody dilution; use positive controls expressing DYNC2LI1 (e.g., kidney or brain lysates) and negative controls (e.g., siRNA knockdown) to confirm specificity.
• In ELISA, this polyclonal antibody may capture native or recombinant DYNC2LI1; pair with a matched detection antibody for sandwich formats if intended.
• Due to cross-reactivity with mouse and rat, it is suitable for comparative studies in rodent models of ciliopathies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents, supporting your development of diagnostic assays targeting ciliary and intraflagellar transport proteins like DYNC2LI1. Whether you need optimized antibodies for ELISA or Western blot applications, or require rare-target raw-material sourcing for ciliopathy research, our team can assist from concept to clinic.