Anti-DYNC2H1 Polyclonal Antibody for WB and ELISA - Q8NCM8

Core Product Specifications and Parameters

Biological Background: DYNC2H1 Function and Localization

• Cytoplasmic dynein 2 heavy chain 1 (DYNC2H1), also known as dynein heavy chain 11 (hDHC11) or dynein heavy chain isotype 1B, is a large motor protein of approximately 493 kDa.
• It is a key component of the dynein-2 complex and functions as a motor for intraflagellar retrograde transport, essential for cilia biogenesis.
• DYNC2H1 may also play roles in intracellular transport between the endoplasmic reticulum and Golgi, as well as in Golgi organization.
• The protein is expressed in multiple isoforms due to alternative splicing.
• Subcellularly, DYNC2H1 localizes to the cytoplasm, cytoskeleton (cilium axoneme), and cell membrane.
• Conserved domains include ATP-binding and nucleotide-binding regions, along with coiled-coil domains characteristic of dynein heavy chains.
• Mutations in DYNC2H1 are associated with ciliopathies, including short-rib thoracic dysplasia and related skeletal disorders, highlighting its developmental importance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-250), which may allow detection of multiple DYNC2H1 isoforms if the epitope is conserved across splice variants.
• Given the large molecular weight (~493 kDa), consider using low-percentage SDS-PAGE gels (e.g., 6% or gradient gels) and extended transfer times for western blotting. Ensure adequate protein loading and use appropriate molecular weight markers for high-range detection.
• For ELISA, the antibody can be used for screening applications; optimize coating and detection conditions based on experimental requirements.
• Cross-reactivity with rat samples is reported; however, performance in other species should be independently validated.
• Inclusion of appropriate positive and negative controls is recommended to confirm specificity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic developers and researchers from concept to clinic. In the area of cytoskeletal and motor proteins, particularly for cilia biology and related disorders, we provide access to validated polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing, including dynein-related tools. Our portfolio and technical consulting are designed to streamline your assay development and procurement needs.