Anti-DYNC1I1 Polyclonal Antibody for WB, IHC-P, ELISA - O14576

Core Product Specifications and Parameters

Biological Background: DYNC1I1 Function and Localization

• Encodes cytoplasmic dynein 1 intermediate chain 1, also known as dynein intermediate chain 1 (DH IC-1), a non-catalytic component of the cytoplasmic dynein 1 motor complex.
• Acts as an accessory component linking dynein to cargos and adapter proteins; specifically mediates binding of dynein to dynactin via the p150-glued (DCTN1) subunit.
• Functions in intracellular retrograde transport of vesicles and organelles along microtubules, and may mediate interaction with membranous organelles and kinetochores.
• Localizes to the cytoplasm and is detected at chromosome centromeres, kinetochores, and spindle poles during cell division.
• Belongs to the dynein intermediate chain family and contains WD repeat domains; subject to post-translational modifications including acetylation and phosphorylation.
• Alternative splicing produces multiple isoforms; implicated in transport and motor protein functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-220 amino acids of human DYNC1I1; this region may be conserved across human, mouse, and rat, consistent with observed cross-reactivity.
• For Western blotting, the expected band is around 73 kDa; validate in relevant cell lysates and consider using positive and negative controls.
• IHC-P may require antigen retrieval and optimization of antibody dilution; nuclear/centrosomal staining patterns should be confirmed with subcellular markers.
• ELISA performance can be assessed with recombinant protein or peptide antigens; adjust coating and detection conditions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with access to a comprehensive range of IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-DYNC1I1 antibody. Covering targets from concept to clinic, we offer one-stop procurement for bulk ancillary reagents and rare target sourcing. For cytoplasmic dynein and motor protein research, CamelBio can support your assay development with tailored raw material solutions.