Anti-DYNC1H1 Rabbit Polyclonal Antibody for WB, ELISA - Q14204

Core Product Specifications and Parameters

Biological Background: DYNC1H1 Function and Localization

• Cytoplasmic dynein 1 heavy chain 1 (DYNC1H1) is a large motor protein that functions as the heavy chain of cytoplasmic dynein 1, a multi-subunit complex responsible for minus-end-directed transport along microtubules.
• DYNC1H1 possesses ATPase activity, generating mechanical force through conformational changes associated with ATP hydrolysis and ADP release, which drives cargo movement.
• It plays a critical role in intracellular retrograde transport of diverse cargoes, including vesicles, organelles, and macromolecular complexes, essential for neuronal function and cell homeostasis.
• DYNC1H1 is involved in mitotic spindle assembly and positioning, facilitating chromosome congression during metaphase and ensuring proper cell division. Related references: PMID:27462074
• The protein is localized to the cytoplasm, associating with the microtubule cytoskeleton, and is enriched at the mitotic spindle during cell division.
• Mutations in DYNC1H1 are linked to neurodegenerative diseases, including Charcot-Marie-Tooth disease type 2O (CMT2O), spinal muscular atrophy with lower extremity predominance (SMALED1), and intellectual disability, underscoring its importance in neuronal development and maintenance.
• DYNC1H1 is subject to post-translational modifications such as acetylation and phosphorylation, which may regulate its activity, cargo binding, and cellular localization.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–210) of human DYNC1H1, which may be critical for specific isoform recognition; verify reactivity in your target tissue or cell line.
• For Western blot (WB), due to the large molecular weight (~532 kDa), use a low-porosity gel (e.g., 4–8% gradient or 5% SDS-PAGE) and ensure thorough transfer; a wet transfer system with extended time is recommended.
• ELISA applications may require optimization of coating antigen and antibody titer; consider using recombinant DYNC1H1 fragments or cell lysates as capture targets.
• The antibody has been validated for human; cross-reactivity with other species should be experimentally confirmed if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated sourcing partner for IVD raw materials, providing one-stop access to high-quality antibodies, antigens, and ancillary reagents for every stage of development. This anti-DYNC1H1 polyclonal antibody supports research into intracellular transport and neurological disorders, and we can assist with bulk orders, custom reagent sourcing, or technical consultation to accelerate your diagnostic assay development.