Anti-Dynamin 3 Rabbit Monoclonal Antibody for WB and ELISA - Q9UQ16

Core Product Specifications and Parameters

Biological Background: Dynamin-3 Function and Localization

• Dynamin-3 (DNM3), also referred to as Dyna III or T-dynamin, is a member of the dynamin GTPase family encoded by the DNM3 gene (Gene ID: 26052). Multiple isoforms are generated through alternative splicing.
• This protein functions as a microtubule-associated force-producing enzyme, capable of bundling microtubules and hydrolyzing GTP. It is primarily implicated in vesicular trafficking, especially endocytosis (By similarity).
• Subcellularly, Dynamin-3 localizes to the cytoplasm and associates with the cytoskeleton, reflecting its role in microtubule dynamics.
• Post-translational modifications such as acetylation and phosphorylation have been reported, potentially modulating its activity or interactions.
• Dynamin-3 contains a conserved GTP-binding domain and is classified as a motor protein and hydrolase, sharing structural features with other dynamins involved in membrane remodeling.
• Through its involvement in cytoskeletal organization and endocytosis, Dynamin-3 contributes to cellular processes like synaptic vesicle recycling and membrane trafficking.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 700–800 of human Dynamin-3, which may confer high target specificity. Confirm performance in your specific assay.
• For Western blotting, the predicted molecular weight of Dynamin-3 is approximately 98 kDa. Consider using a 7.5–10% SDS-PAGE gel and optimized transfer conditions for large proteins.
• The antibody is suitable for ELISA; however, optimal coating concentrations and secondary antibody dilutions should be empirically determined.
• Reported cross-reactivity with human and mouse samples suggests utility in these species; validate in other species as needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive selection of IVD raw materials, including validated monoclonal and polyclonal antibodies, antibody pairs, and ancillary reagents. This Dynamin-3 antibody supports research in cytoskeletal dynamics and endocytosis, areas critical for diagnostic development. Our team can assist with sourcing rare-target raw materials and provide end-to-end support from concept to clinic.