Anti-DVL2 Rabbit Polyclonal Antibody for IF/ICC and ELISA - O14641

Core Product Specifications and Parameters

Biological Background: DVL2 Function and Localization

• DVL2 plays a crucial role in signal transduction pathways mediated by multiple Wnt genes. Related references: PMID:24616100
• It functions in both canonical and non-canonical Wnt signaling by binding to the cytoplasmic tail of Frizzled family receptors and transmitting signals to downstream effectors.
• DVL2 facilitates the internalization and degradation of Frizzled proteins upon Wnt stimulation, thus regulating receptor availability.
• The protein localizes to multiple cellular compartments including the cell membrane, cytoplasm, cytosol, cytoplasmic vesicles, and nucleus, reflecting its dynamic signaling roles.
• DVL2 undergoes post-translational modifications such as phosphorylation, isopeptide bond formation, and ubiquitin-like (Ubl) conjugation, which modulate its activity and stability.
• As a developmental protein, DVL2 is implicated in key processes like cell fate specification and tissue patterning.

Experimental Guidance and Technical Tips

• The immunogen targets the N-terminal region (aa 1–240) containing the DIX domain, which is critical for DVL2 polymerization and Wnt signalosome formation; this design may favor detection of full-length endogenous protein.
• For IF/ICC, carefully optimize fixation and permeabilization protocols to preserve the diverse subcellular localizations of DVL2 (membrane, cytoplasm, vesicles, nucleus) which can change upon Wnt stimulation.
• In ELISA, the polyclonal nature of this antibody may provide robust signal, but potential cross-reactivity with other DVL family members should be validated in the relevant sample system.
• As a polyclonal antibody, lot-to-lot variability is possible; include appropriate positive and negative controls in each experiment to ensure reproducibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a trusted one-stop partner for IVD raw materials, supporting your Wnt signaling research and diagnostic development with high-quality reagents like this anti-DVL2 polyclonal antibody. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, and can assist in sourcing rare-target raw materials to streamline your IVD workflow from discovery to commercialization.