Anti-DVL1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O14640

Core Product Specifications and Parameters

Biological Background: DVL1 Function and Localization

• DVL1 is a segment polarity protein dishevelled homolog, also known as DSH homolog 1, encoded by the DVL1 gene. It is a key component of the Wnt signaling pathway.
• Participates in both canonical and non-canonical Wnt signaling by binding to the cytoplasmic C-terminus of frizzled receptors and transducing signals to downstream effectors.
• Mediates LEF1 activation in response to WNT1 and WNT3A, contributing to transcriptional regulation of Wnt target genes.
• Forms a ternary complex with PAK1 and MUSK that is critical for MUSK-dependent AChR clustering during neuromuscular junction formation.
• Localizes to the cell membrane, cytoplasm, cytosol, and cytoplasmic vesicles, enabling dynamic signaling from receptor complexes to intracellular effectors.
• Post-translationally modified by phosphorylation and ubiquitination (as indicated by keywords), which likely regulate its activity and stability.
• Alternative splicing generates multiple isoforms, potentially contributing to functional diversity in different tissues.
• Associated with developmental processes and human disease (e.g., dwarfism, based on keyword annotation).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 510-640) of human DVL1; therefore, the antibody may detect full-length DVL1 and any C-terminal fragments.
• For Western blot (WB), the expected molecular weight is approximately 75 kDa; alternative splicing may produce additional bands. Consider using appropriate positive controls such as HeLa or HEK293 cell lysates.
• In immunohistochemistry (IHC-P), antigen retrieval may be necessary; validate the staining pattern in tissues known to express DVL1, such as brain or muscle.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization conditions to preserve the native localization (cell membrane, cytoplasm, vesicles).
• ELISA applications may be optimized using recombinant DVL1 protein as a standard. Consider cross-reactivity when using mouse or rat samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner for diagnostic manufacturers, laboratories, and research institutions. This DVL1 polyclonal antibody supports investigations into Wnt signaling, neuromuscular junction biology, and related disease research. Beyond this product, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw materials to accelerate your projects from concept to clinic.