Anti-DUT Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P33316

Core Product Specifications and Parameters

Biological Background: DUT Function and Localization

• DUT encodes deoxyuridine 5'-triphosphate nucleotidohydrolase, a mitochondrial and nuclear enzyme that hydrolyzes dUTP to dUMP and pyrophosphate (dUTP pyrophosphatase). Related references: PMID:17880943 PMID:8631816 PMID:8805593
• By cleaving dUTP, DUT prevents uracil misincorporation into DNA and supplies dUMP for de novo thymidylate biosynthesis, critical for DNA replication fidelity and genome integrity.
• DUT also acts as a negative regulator of peroxisome proliferator-activated receptor (PPAR) through its N-terminal domain, inhibiting PPAR dimerization with retinoid X receptor (By similarity).
• The protein is essential for embryonic development, as shown by functional studies (By similarity).
• Subcellular localization includes both nucleus and mitochondrion, with a transit peptide directing mitochondrial import.
• Tissue specificity: Isoform 3 is constitutively expressed, while isoform 2 expression is replication-dependent and its degradation coincides with cessation of DNA replication (at protein level).
• Keywords: Alternative splicing, Diabetes mellitus, Disease variant, Hydrolase, Magnesium, Nucleotide metabolism, Nucleus, Phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen sequence (amino acids 89–252) covers the catalytic domain of mature DUT; when using this antibody in western blotting, consider validating the detected band at approximately 27 kDa in the relevant sample system and account for possible isoform-specific migration.
• For immunohistochemistry (IHC‑P) and immunofluorescence (IF/ICC), optimize antigen retrieval and permeabilization conditions as needed for your tissue or cell type; the nuclear and mitochondrial localization may require appropriate controls.
• In ELISA applications, include proper positive and negative controls and consider the polyclonal nature of the antibody when designing sensitive detection formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated IVD raw-material sourcing partner, offering validated antibody pairs, high‑quality monoclonal and polyclonal antibodies, and bulk ancillary reagents to accelerate diagnostic development. For research on nucleotide metabolism, DNA replication, and genomic stability, we provide DUT antibodies and related reagents to streamline your assay workflows. Contact us for customized one‑stop sourcing solutions that support your projects from concept to clinic.