Anti-DUSP9 (MKP-4) Polyclonal Antibody for WB, IF/ICC, ELISA - Q99956

Core Product Specifications and Parameters

Biological Background: DUSP9 Function and Localization

• DUSP9 encodes a dual specificity protein phosphatase that belongs to the MAP kinase phosphatase (MKP) family, specifically designated as MKP-4.
• It dephosphorylates and inactivates MAP kinases, with a strong preference for the ERK (extracellular signal-regulated kinase) subfamily, thereby negatively regulating ERK signaling cascades.
• DUSP9 is localized in the cytoplasm, where it encounters activated ERK kinases to mediate signal attenuation.
• The protein is classified as a phosphoprotein, indicating that its activity or stability may be regulated by phosphorylation.
• Structural studies (keyword: 3D-structure) have determined the three-dimensional arrangement of DUSP9, providing insights into its catalytic mechanism and substrate recognition.
• Keywords such as Hydrolase, Protein phosphatase, and Reference proteome highlight its enzymatic function and curated annotation status.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–200) of human DUSP9; the resulting polyclonal antibody may recognize both phosphorylated and unphosphorylated forms. Consider this when probing samples with varying phosphatase activity.
• For western blot (WB), a band at ~42 kDa is expected in human and mouse lysates. Verify specificity using appropriate controls (e.g., ERK activation or knockdown models).
• In immunofluorescence (IF/ICC), anticipate cytoplasmic staining consistent with DUSP9 localization. Co-staining with ERK markers can validate functional context.
• ELISA applications require optimization of coating antigen and detection conditions; cross-reactivity with mouse DUSP9 enables comparative studies.
• When working with phosphatase inhibitors, be aware that they may alter DUSP9 phosphorylation status and antibody binding.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material sourcing partner, CamelBio supports diagnostic manufacturers and researchers with validated antibody pairs and optimized polyclonal antibodies like this anti-DUSP9 pAb. We also supply bulk ancillary reagents and excel in sourcing rare targets, ensuring you have the tools to advance ERK signaling studies and phosphatase-based diagnostics from concept to clinic.