Anti-DUSP8 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q13202

Core Product Specifications and Parameters

Biological Background: DUSP8 Function and Localization

• DUSP8 (Dual specificity protein phosphatase 8), also known as hVH-5, is a member of the dual specificity phosphatase (DUSP) family that can dephosphorylate phosphotyrosine, phosphoserine, and phosphothreonine residues.
• It negatively regulates mitogen-activated protein kinase (MAPK) signaling pathways by catalyzing the dephosphorylation of activated MAP kinases, thus controlling cellular processes such as proliferation and differentiation.
• DUSP8 is predominantly localized in both the cytoplasm and the nucleus, allowing it to modulate signaling cascades in different cellular compartments.
• Expression is abundant in brain, heart, and skeletal muscle, suggesting a role in tissue-specific signaling regulation.
• Structural studies have provided insights into its catalytic mechanism (3D-structure available in the PDB).

Experimental Guidance and Technical Tips

• The antibody was raised against a peptide from the N-terminal region (amino acids 1–100) of human DUSP8, ensuring recognition of the full-length protein; consider sequence alignment if working with other species.
• For western blot (WB), a band at approximately 66 kDa is expected; use appropriate positive controls (e.g., brain, heart, or skeletal muscle lysates).
• In immunofluorescence (IF/ICC), both cytoplasmic and nuclear staining may be observed; optimize fixation and permeabilization for target localization.
• ELISA performance can be verified by coating with the immunogen peptide; cross-reactivity with other DUSP family members cannot be excluded, so validate in the relevant sample system.
• The antibody shows cross-reactivity with mouse and rat as listed, but users should verify reactivity in their specific experimental models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This DUSP8 polyclonal antibody supports research into MAPK signaling and phosphatase activity, with cross-species applicability. For specialized or rare-target raw materials, CamelBio’s sourcing network can help bridge your development needs from concept to clinic.