Anti-DUSP6 Rabbit Monoclonal Antibody for WB, IP, IHC-P, ELISA - Q16828

Core Product Specifications and Parameters

Biological Background: DUSP6 Function and Localization

• DUSP6 is a dual specificity protein phosphatase that mediates the dephosphorylation and inactivation of MAP kinases, exhibiting high specificity for the ERK family (MAPK1/3). Related references: PMID:8670865
• The enzyme plays a crucial role in the resolution of chronic postoperative pain by dephosphorylating spinal MAPK1/3 and MAP kinase p38, and contributes to acute pain resolution in wound tissue.
• DUSP6 promotes cell differentiation by regulating MAPK1/MAPK3 activity and controlling the expression of AP1 transcription factors. Related references: PMID:29043977
• The protein is predominantly localized to the cytoplasm.
• DUSP6 is expressed in keratinocytes at the protein level.
• Post-translational modifications include ubiquitin conjugation.
• Disease-associated variants have been linked to hypogonadotropic hypogonadism and Kallmann syndrome.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to a synthetic peptide spanning amino acids 282–381 of human DUSP6, a region conserved in mouse and rat, which aligns with the observed cross-reactivity.
• For Western blot (WB) analysis, a specific band at approximately 42 kDa is expected; consider using appropriate positive controls such as lysates from keratinocytes or ERK-signaling active cell lines.
• In immunohistochemistry (IHC-P), validate staining in tissues known to express DUSP6, like skin (keratinocytes) or neural tissues, with attention to cytoplasmic localization.
• When used in immunoprecipitation (IP) or ELISA, optimize antibody concentrations and buffer conditions; cross-reactivity with mouse and rat samples may require species-specific validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive one-stop access to IVD raw materials, technical services, and consulting for diagnostic manufacturers and research institutes. For targets involved in MAP kinase signaling and phosphatase activity, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to streamline your assay development. Whether you need rare target raw-material sourcing or support from concept to clinic, CamelBio bridges your procurement needs with quality and efficiency.