Anti-DUSP6 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q16828

Core Product Specifications and Parameters

Biological Background: DUSP6 Function and Localization

• DUSP6 (also known as MKP-3 or PYST1) is a dual-specificity protein phosphatase that specifically mediates dephosphorylation and inactivation of ERK family MAP kinases (MAPK1/MAPK3). Related references: PMID:8670865
• It plays a crucial role in the resolution of chronic postoperative pain by dephosphorylating spinal MAPK1/3 and p38, thereby alleviating acute allodynia after peripheral surgery.
• DUSP6 promotes cell differentiation through regulation of MAPK1/MAPK3 activity and downstream AP1 transcription factor expression. Related references: PMID:29043977
• The protein is primarily localized to the cytoplasm.
• DUSP6 is expressed in keratinocytes (at protein level) and may have tissue-specific regulatory roles.
• It belongs to the dual-specificity phosphatase family and is subject to alternative splicing. Disease-associated variants have been linked to hypogonadotropic hypogonadism and Kallmann syndrome.
• Additional features include Ubl conjugation, a known 3D-structure, and inclusion in reference proteome databases.

Experimental Guidance and Technical Tips

• For western blotting, a band at approximately 42 kDa is expected. Consider using positive control lysates from HeLa, Jurkat, or keratinocyte cell lines to validate specificity.
• In IHC-P, antigen retrieval conditions may need optimization for formalin-fixed paraffin-embedded sections; test on human tissues expressing DUSP6.
• For IF/ICC, proper fixation and permeabilization are critical due to the cytoplasmic localization of DUSP6.
• The antibody cross-reacts with mouse and rat, making it suitable for rodent model studies; validate in the relevant sample system.
• ELISA can be used for quantitative detection; consider pairing with a matched detection antibody for sandwich ELISA setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials for MAPK signaling and pain research, including this DUSP6-specific polyclonal antibody. We offer validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support your development from concept to clinic.