Anti-DUSP5 Rabbit Polyclonal Antibody for WB and ELISA - Q16690

Core Product Specifications and Parameters

Biological Background: DUSP5 Function and Localization

• DUSP5 (dual specificity protein phosphatase 5), also known as hVH3, is a member of the dual specificity phosphatase family that can hydrolyze phosphate groups from phosphotyrosine, phosphoserine, and phosphothreonine residues.
• The enzyme exhibits the highest relative activity toward ERK1, suggesting a role in mitogen-activated protein kinase (MAPK) signal regulation.
• Encoded by the DUSP5 gene located at chromosome 10q25.2, this protein is conserved across species and is classified under protein phosphatases (EC 3.1.3.48) and hydrolases.
• Subcellularly, DUSP5 localizes to the nucleus, consistent with its function in modulating nuclear ERK signaling.
• The protein has been characterized structurally (3D-structure available) and is part of the reference proteome, underscoring its experimental and diagnostic relevance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 315–384) of human DUSP5; consider that this region may be shared among splice variants if any exist in the target species.
• For Western blotting, DUSP5 migrates at a predicted molecular weight of ~42 kDa; validate using appropriate positive controls (e.g., HeLa or HEK293 cell lysates) and adjust blocking conditions to minimize background.
• Nuclear enrichment protocols could enhance detection sensitivity given the nuclear localization of DUSP5.
• In ELISA applications, confirm coating and detection conditions using recombinant DUSP5 protein as a standard, and assess cross-reactivity with related phosphatase family members if necessary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and researchers, providing a wide array of validated antibodies, bulk reagents, and customized raw-material solutions. For phosphatase-targeted assay development, including DUSP5, we offer polyclonal antibodies optimized for detection, along with ancillary reagents and technical consultation to bridge the gap from development to clinical application. Whether you require established antibody pairs or assistance with rare target procurement, CamelBio is dedicated to supporting your diagnostic innovation efficiently.