Anti-DUSP22 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NRW4

Core Product Specifications and Parameters

Biological Background: DUSP22 Function and Localization

• DUSP22, also known as JKAP, JSP-1, LMW-DSP2, and MKP-x, is a dual specificity phosphatase that can dephosphorylate both phosphotyrosine and phosphoserine/threonine residues. Related references: PMID:24714587, PMID:38225265
• Activates the JNK signaling pathway, playing a role in cellular stress responses. Related references: PMID:11717427
• Inhibits T-cell receptor (TCR) signaling and T-cell mediated immune responses by dephosphorylating and inactivating the tyrosine kinase LCK, and by inducing proteasomal degradation of the E3 ubiquitin ligase UBR2. Related references: PMID:24714587, PMID:38225265
• May also modulate B-cell receptor signaling and B-cell function.
• Localizes primarily to the cytoplasm, consistent with its role in signal transduction cascades.
• Ubiquitously expressed, with highest levels in heart, placenta, lung, liver, kidney, and pancreas, indicating broad regulatory functions.
• Post-translationally modified by myristoylation and phosphorylation (as inferred from UniProt keywords), which may regulate its activity and localization.
• Belongs to the dual specificity protein phosphatase family, characterized by their ability to regulate MAP kinase signaling pathways.

Experimental Guidance and Technical Tips

• The immunogen used covers amino acids 1-184 of human DUSP22, suggesting the antibody may recognize the N-terminal region. For WB, consider a dilution range and validate with appropriate positive controls (e.g., DUSP22-expressing cell lines).
• As a polyclonal antibody, robust signal amplification is expected, but careful blocking and optimization may be needed to minimize background in IF/ICC.
• For ELISA, ensure optimal coating conditions and antigen presentation; consider testing with recombinant protein or tissue lysates from human samples.
• Verify cross-reactivity in your specific experimental system; the antibody is validated for human samples, and performance in other species is not guaranteed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing bridge for IVD raw materials, connecting diagnostic manufacturers and research teams with validated antibodies and reagents. This anti-DUSP22 polyclonal antibody supports investigations into JNK signaling and immune regulation, critical for developing assays in autoimmune and cancer diagnostics. Contact CamelBio for bulk procurement, customized antibody solutions, and one-stop support from concept to clinic.