Anti-DUSP1/MKP1 Polyclonal Antibody for WB, ELISA - P28562

Core Product Specifications and Parameters

Biological Background: DUSP1/MKP1 Function and Localization

• DUSP1 (dual specificity phosphatase 1), also known as MAP kinase phosphatase 1 (MKP-1), hVH1, or CL100, is a nuclear dual-specificity protein phosphatase that acts on phosphotyrosine and phosphothreonine residues.
• It specifically dephosphorylates MAP kinase MAPK1/ERK2 on both Thr-183 and Tyr-185, thereby negatively regulating its activity and playing a key role in the control of the cell cycle, particularly during meiotic processes.
• The protein is primarily localized in the nucleus, consistent with its role in modulating kinase signaling cascades that control gene expression and cell proliferation.
• DUSP1 expression exhibits tissue specificity: high levels are detected in lung, liver, placenta, and pancreas; moderate levels in heart and skeletal muscle; and lower levels in brain and kidney.
• Post-translational modifications include phosphorylation and ubiquitin conjugation, which may regulate its stability and activity under various stress conditions.
• Functional annotation keywords highlight its involvement in cell cycle regulation, stress response, and protein dephosphorylation, and it belongs to the dual-specificity phosphatase family.

Experimental Guidance and Technical Tips

• For Western blotting, a band of approximately 39–40 kDa is expected; consider using reducing conditions and appropriate molecular weight markers for confirmation.
• When using this polyclonal antibody in ELISA, validate optimal coating and detection conditions within the relevant sample system, and include appropriate controls to account for polyclonal heterogeneity.
• Given the conserved nature of the target, the antibody’s cross-reactivity with mouse and rat samples can be expected, but it is advisable to verify performance in your specific model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a reliable source of IVD raw materials, including this anti-DUSP1/MKP1 antibody, which serves as a critical tool for studying MAP kinase signaling and stress pathways. In addition to validated antibodies, CamelBio offers a comprehensive portfolio of ancillary reagents and can assist in sourcing specialized raw materials for upstream and downstream assay development. Leveraging a broad supply network, CamelBio supports projects from early R&D through to clinical application, delivering tailored solutions for your IVD needs.