Anti-DUSP16 Rabbit Polyclonal Antibody for WB, ELISA - Q9BY84

Core Product Specifications and Parameters

Biological Background: Dual specificity protein phosphatase 16 Function and Localization

• Dual specificity protein phosphatase 16 (DUSP16), also known as MAP kinase phosphatase 7 (MKP-7) or MKP7, is a member of the dual specificity phosphatase family.
• DUSP16 functions as a MAP kinase phosphatase, inactivating mitogen-activated protein kinases (MAPKs) through dephosphorylation.
• The enzyme specifically dephosphorylates MAPK10 (JNK3) when bound to the scaffold protein ARRB2 (beta-arrestin-2).
• DUSP16 exhibits dual specificity, capable of hydrolyzing phospho-tyrosine and phospho-serine/threonine residues.
• The protein localizes to the cytoplasm, nucleus, and cytoplasmic vesicles, indicating its involvement in multiple subcellular signaling compartments.
• Post-translational modifications include acetylation and phosphorylation, as documented in proteomic datasets.
• Alternative splicing of the DUSP16 gene generates multiple isoforms, which may influence its regulatory functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 416–665 of human DUSP16, a region that may contain conserved epitopes, potentially contributing to cross-reactivity with mouse and rat orthologs.
• For Western blot, consider testing a range of antibody dilutions and validate in the relevant species and tissue/cell type; the predicted molecular weight is approximately 73 kDa.
• For ELISA, optimize antigen coating and antibody concentrations to achieve the best signal-to-noise ratio.
• As a polyclonal antibody, it may recognize multiple isoforms or modified forms; confirm specificity for the target in your experimental system using appropriate controls (e.g., immunogen blocking).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop partner for IVD raw materials, providing reliable access to validated antibody reagents like this anti-DUSP16 polyclonal antibody for cell signaling research. We offer a broad portfolio of antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents to support your assay development from concept to testing. For specialized or rare target sourcing needs, our team can assist with customized solutions to accelerate your projects.