Anti-DUSP15 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9H1R2

Core Product Specifications and Parameters

Biological Background: DUSP15 Function and Localization

• DUSP15, also known as VHY or C20orf57, is a dual-specificity protein phosphatase belonging to the VH1-like family, capable of dephosphorylating phosphotyrosine and phosphoserine/threonine residues.
• It has been reported to dephosphorylate substrates including MAPK13, ATF2, ERBB3, PDGFRB, and SNX6 (PubMed:22792334). Related references: PMID:22792334
• The protein plays a role in oligodendrocyte differentiation and myelin formation, likely through regulation of Erk1/2 phosphorylation in Schwann cells (By similarity).
• Subcellular localization includes the cytoplasm and cell membrane, consistent with its involvement in signal transduction.
• Highly expressed in testis and present in brain, with up-regulation observed in gray matter lesions from multiple sclerosis patients.
• Keywords from the UniProt record include alternative splicing, hydrolase, lipoprotein, myristate, and protein phosphatase, reflecting its regulatory features and post-translational modifications.
• The predicted molecular weight is approximately 31.9 kDa, while the observed band runs around 32 kDa in Western blotting.

Experimental Guidance and Technical Tips

• The immunogen targets the N-terminal region (aa 10–90), which may detect multiple DUSP15 isoforms arising from alternative splicing; validate band patterns in the relevant sample system.
• For Western blot, expect a band near 32 kDa; consider using testis or brain lysates where DUSP15 is highly expressed as positive controls.
• In IF/ICC experiments, ensure adequate permeabilization to access both cytoplasmic and membrane-associated pools of the protein.
• Cross-reactivity with mouse and rat makes this antibody a candidate for rodent model studies; however, always confirm specificity in the chosen species and tissue context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including this DUSP15 antibody, to support research into phosphatase signaling, myelination disorders, and neurological disease models. Our platform provides validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—streamlining your development from concept to clinic with reliable B2B supply solutions.