Anti-DUSP14 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O95147

Core Product Specifications and Parameters

Biological Background: DUSP14 Function and Localization

• DUSP14, also known as MKP6 or MKP-L, is a dual-specificity protein phosphatase that belongs to the MAP kinase phosphatase family.
• It functions as a negative regulator of mitogen-activated protein kinase (MAPK) signaling by dephosphorylating ERK, JNK, and p38 MAP kinases.
• In T-cell receptor (TCR) signaling, DUSP14 plays a negative role by dephosphorylating the MAP3K7 adapter TAB1, leading to its inactivation. Related references: PMID:24403530
• The protein is classified under hydrolase and protein phosphatase activities, and is a member of the reference proteome for human studies.
• Its molecular weight is approximately 22.3 kDa (calculated), consistent with the reported 22 kDa band in western blot applications.

Experimental Guidance and Technical Tips

• For western blot, a band at ~22 kDa is expected. Validate with appropriate positive and negative controls.
• For immunofluorescence/ICC, optimize fixation and permeabilization conditions to detect endogenous DUSP14, as its subcellular localization may be context-dependent.
• The polyclonal nature of the antibody may result in recognition of multiple epitopes, reducing the risk of signal loss due to post-translational modifications.
• Since the immunogen is nearly full-length, the antibody is likely to detect total DUSP14, but confirm specificity by siRNA knockdown or knockout models in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for IVD raw materials, offering a comprehensive selection of validated antibodies, including this anti-DUSP14 polyclonal antibody for MAP kinase signaling research. We supply high-quality IVD-grade reagents such as antibody pairs, monoclonal and polyclonal antibodies, and ancillary bulk materials to support diagnostic development. Our expertise extends to rare target sourcing, helping you secure critical components from research to clinical application.