Anti-DTYMK Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P23919

Core Product Specifications and Parameters

Biological Background: Thymidylate Kinase Function and Localization

• Thymidylate kinase (dTMP kinase), encoded by the DTYMK gene, is a nucleoside monophosphate kinase that plays a central role in pyrimidine metabolism.
• It catalyzes the ATP-dependent phosphorylation of thymidine monophosphate (dTMP) to thymidine diphosphate (dTDP) in the presence of Mg²⁺, a critical step in the de novo biosynthesis of dTTP, an essential DNA precursor.
• The enzyme is classified as a kinase and transferase, utilizing ATP as the phosphate donor and binding nucleotides for catalysis.
• DTYMK is reported to undergo alternative splicing, generating multiple protein isoforms that may have distinct regulatory or functional properties.
• Post-translational modifications include acetylation, which could modulate enzyme activity, stability, or subcellular localization.
• Clinically, DTYMK is associated with disease variants linked to intellectual disability and neurodegeneration, underscoring its importance in nucleotide homeostasis for neural development and function.

Experimental Guidance and Technical Tips

• The immunogen comprises a recombinant protein corresponding to the full-length human DTYMK (amino acids 1–212), making the antibody likely to recognize the native protein across species.
• For Western Blot, an expected band around 24 kDa should be observed; include appropriate positive and negative controls to confirm specificity.
• In IF/ICC experiments, fixation and permeabilization conditions may influence staining; optimization with known positive cells (e.g., human cell lines) is recommended.
• ELISA detection can be performed using standard protocols; consider titrating the antibody to determine the optimal working concentration.
• Cross-reactivity with mouse and rat DTYMK has been reported, suggesting recognition of conserved epitopes; validate the antibody in the relevant sample system before routine use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, bulk ancillary reagents, and access to rare target antibodies like this DTYMK polyclonal. Whether you need specialized immunoreagents for nucleotide metabolism studies or broader assay development, CamelBio streamlines procurement from concept to clinic, supporting diagnostic manufacturers, labs, and research institutes with reliable supply and technical consultation.