Anti-DTYMK Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P23919

Core Product Specifications and Parameters

Biological Background: DTYMK Function and Localization

• DTYMK (Thymidylate kinase), also known as dTMP kinase, is an enzyme central to the pyrimidine salvage pathway, catalyzing the ATP-dependent phosphorylation of dTMP to dTDP, a direct precursor of dTTP required for DNA synthesis.
• The kinase activity strictly depends on the presence of Mg²⁺ and preferentially uses ATP as the phosphate donor, ensuring balanced dNTP pools.
• Alternative splicing of the DTYMK gene generates multiple transcript variants, though the full-length isoform is predominantly studied.
• Post-translational acetylation of DTYMK has been identified by proteomic studies, suggesting regulatory modification.
• Keywords from UniProt record: 3D-structure, Acetylation, Alternative splicing, ATP-binding, Disease variant, Intellectual disability, Kinase, Neurodegeneration, Nucleotide biosynthesis, Nucleotide-binding, Proteomics identification, Reference proteome, Transferase.
• Structurally, the protein contains a nucleotide-binding domain and has been characterized by X-ray crystallography (3D structure available).
• Disease-associated mutations in DTYMK have been linked to intellectual disability and neurodegenerative disorders, underscoring its importance in neurological development.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the internal region (aa 100-200) of human DTYMK, which can produce antibodies recognizing full-length endogenous protein.
• For Western blot (WB), a band at approximately 24 kDa is expected in human cell lysates; consider using reducing conditions and validate in relevant human tissues or cell lines.
• For immunofluorescence (IF/ICC), standard fixation (e.g., 4% PFA) and permeabilization (e.g., 0.1% Triton X-100) protocols are likely suitable; optimize primary antibody dilution based on endogenous expression levels.
• For ELISA, direct or indirect formats can be employed; validate specificity using recombinant DTYMK protein or peptide competition assays.
• Since the antibody is rabbit monoclonal, it offers high batch-to-batch consistency, beneficial for quantitative studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering validated monoclonal antibodies, such as this DTYMK rabbit mAb, for nucleotide metabolism research and diagnostic assay development. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your projects from concept to clinic. For rare targets like DTYMK, CamelBio provides reliable sourcing and technical guidance, ensuring you receive consistent, high-quality materials.