Anti-DTNPB1 Rabbit Polyclonal Antibody for WB, ELISA - Q96EV8

Core Product Specifications and Parameters

Biological Background: Dysbindin Function and Localization

• Dysbindin (dystrobrevin-binding protein 1), also known as BLOC-1 subunit 8 or Hermansky-Pudlak syndrome 7 protein (HPS7), is encoded by the DTNBP1 gene in humans.
• It is a core component of the biogenesis of lysosome-related organelles complex 1 (BLOC-1), which is essential for the normal biogenesis of LROs such as platelet dense granules and melanosomes.
• Dysbindin functions in membrane protein cargo sorting into vesicles at the cell body for delivery to neurites and nerve terminals, working together with the AP-3 complex.
• It is involved in synaptic vesicle trafficking and neurotransmitter release, and regulates cell surface exposure of the dopamine D2 receptor (DRD2).
• The protein localizes to multiple compartments including the cytoplasm, endosome membranes, melanosome membranes, postsynaptic density, endoplasmic reticulum, nucleus, and synaptic vesicle membranes.
• In the brain, dysbindin is detected in neurons and neuropil, with isoform 1 expressed in the cerebral cortex and hippocampal formation, showing highest levels in hippocampal CA2, CA3, and dentate gyrus.
• Keywords associated with dysbindin include Hermansky-Pudlak syndrome, schizophrenia, albinism, sensory transduction, and synaptic function.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a recombinant protein covering amino acids 1-351 of human DTNBP1 (NP_115498.2); consider this full-length fragment when designing confirmation experiments.
• For Western blot, the predicted molecular weight of dysbindin is approximately 39 kDa; validate band detection in relevant sample systems such as brain tissue lysates.
• ELISA application is suggested based on the supplier’s testing; verify optimal coating and detection conditions using purified dysbindin or positive control lysates.
• Cross-reactivity with mouse has been indicated; when working with mouse samples, confirm specificity by alignment of the immunogen sequence with the mouse ortholog.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and researchers with a comprehensive portfolio of IVD raw materials. This anti-DTNPB1 polyclonal antibody supports neuroscience and lysosome-related organelle research, and can be complemented by CamelBio’s bulk ancillary reagents, validated antibody pairs, and rare-target sourcing capabilities to streamline your assay development from concept to commercialization.