Anti-DTH8 Rabbit Polyclonal Antibody for WB and ELISA - Q0J7P4

Core Product Specifications and Parameters

Biological Background: DTH8 Function and Localization

• DTH8, also known as Nuclear transcription factor Y subunit B-11 (NF-YB11), is a transcriptional regulator that functions as a flowering repressor under long day conditions in rice (Oryza sativa). Related references: PMID:20566706 PMID:21148627
• It acts by negatively regulating the expression of floral pathway integrators EHD1 and HD3A, thereby delaying heading date. Related references: PMID:20566706 PMID:21148627
• DTH8 is the rice ortholog of the Arabidopsis HAP3 subunit and belongs to the NF-YB family of transcription factors, forming part of the heterotrimeric NF-Y/HAP complex that binds CCAAT motifs.
• Beyond flowering time control, DTH8 also modulates plant height by promoting internode cell elongation, linking developmental timing with morphological traits. Related references: PMID:20566706
• The protein is localized to both the nucleus and the cytoplasm, consistent with its role as a transcription factor that may shuttle between compartments.
• Tissue expression analysis indicates that DTH8 is present in multiple tissues, including roots, culms, nodes, leaf blades, leaf sheaths, and young panicles, suggesting broad regulatory functions.
• The molecular weight of the DTH8 protein is approximately 30 kDa (predicted 29.8 kDa), and it is annotated with functional keywords such as DNA-binding, flowering, growth regulation, and transcription regulation.

Experimental Guidance and Technical Tips

• The antibody was raised against recombinant Oryza sativa DTH8 protein; it is expected to detect endogenous DTH8 in rice by WB and ELISA. Validate detection in known expressing tissues (e.g., leaf blades, young panicles) under appropriate conditions.
• For WB, consider testing a dilution series on rice lysates and include positive controls such as recombinant immunogen or tissue samples with confirmed DTH8 expression.
• In ELISA, the recombinant DTH8 protein can serve as a positive coating antigen; perform cross-reactivity checks with other NF-YB family members if needed.
• Polyclonal antibodies may recognize multiple epitopes; to confirm specificity, consider using knockdown or knockout rice lines, or pre-adsorption with the immunogen.
• Given DTH8's nuclear-cytoplasmic localization, subcellular fractionation could be employed for localization studies, but the antibody's suitability for such applications should be experimentally determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive, one-stop sourcing of IVD raw materials for diagnostic manufacturers, laboratories, and research institutes. For rice developmental biology and flowering time research, we supply validated polyclonal and monoclonal antibodies, including anti-DTH8 reagents, along with bulk ancillary reagents and technical consulting. Our portfolio covers validated antibody pairs, optimized antibodies, and rare-target sourcing, streamlining your path from concept to clinic.