Anti-DSPP Polyclonal Antibody for WB, ELISA - Q9NZW4

Core Product Specifications and Parameters

Biological Background: DSPP Function and Localization

• Dentin sialophosphoprotein (DSPP) is a secreted extracellular matrix protein predominantly expressed in teeth, where it is synthesized by odontoblasts and transiently by pre‑ameloblasts.
• DSPP is proteolytically processed into two functional subunits: dentin sialoprotein (DSP) and dentin phosphoprotein (DPP). DSP promotes dentinogenesis, while DPP binds high concentrations of calcium, facilitates initial mineralization of dentin collagen, and regulates crystal size and shape.
• Belongs to the SIBLING (Small Integrin‑Binding Ligand N‑linked Glycoprotein) family, a group of acidic phosphoproteins critical for biomineralization.
• Subcellular location is the extracellular space and extracellular matrix of dentin and bone.
• Post‑translational modifications include N‑ and O‑linked glycosylation, sialylation, and extensive serine/aspartic acid phosphorylation, conferring a highly acidic character essential for calcium interactions.
• Mutations in DSPP are associated with dentinogenesis imperfecta and autosomal dominant non‑syndromic sensorineural hearing loss (DFNA39), underscoring its roles in dentin and auditory system development.
• Keywords: Biomineralization, Calcium, Deafness, Disease variant, Extracellular matrix, Glycoprotein, Phosphoprotein, Reference proteome, Secreted, Signal.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the DSP region (amino acids 16‑146); thus, the antibody primarily recognizes DSP but may also detect the full‑length precursor depending on sample context.
• For Western blot, anticipate bands at ~95 kDa (DSP), ~45‑55 kDa (DPP fragments), and/or the ~131 kDa full‑length protein; validate sizes with molecular weight markers and appropriate positive controls.
• Use tooth, osteoblast, or odontoblast‑derived lysates as positive controls; for cross‑species detection, verify with mouse or rat dental tissue extracts.
• For ELISA, coat plates with recombinant DSPP or a synthetic peptide containing the immunogen sequence and optimize coating and blocking conditions.
• As a polyclonal antibody, consider additional blocking steps (e.g., 5% non‑fat milk or BSA) and secondary antibody titration to minimize non‑specific binding.
• Always include a no‑primary‑antibody control to confirm signal specificity.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research groups with a streamlined supply chain for IVD raw materials, including this DSPP polyclonal antibody tailored for biomineralization and dental disorder studies. Our portfolio covers validated antibody pairs, bulk monoclonal/polyclonal antibodies, ancillary reagents, and customized sourcing for rare‑target raw materials, supporting you from discovery to clinical application.