Anti-DSG3/Desmoglein-3 Rabbit Polyclonal Antibody for IHC-P, ELISA - P32926

Core Product Specifications and Parameters

Biological Background: Desmoglein-3 Function and Localization

• Desmoglein-3 (DSG3), also known as 130 kDa pemphigus vulgaris antigen (PVA) and cadherin family member 6 (CDHF6), is a calcium-binding transmembrane glycoprotein of the cadherin superfamily, primarily involved in desmosome-mediated cell-cell adhesion.

• DSG3 is a core component of desmosome cell-cell junctions and is required for positive regulation of cellular adhesion and for adherens and desmosome junction assembly in response to mechanical force in keratinocytes. Related references: PMID:31835537

• Essential for desmosome-mediated cell-cell adhesion of cells surrounding the telogen hair club and the basal layer of the outer root sheath epithelium, thereby anchoring telogen hairs in the hair follicle. Related references: PMID:9701552

• Required for the maintenance of the epithelial barrier by promoting desmosome-mediated intercellular attachment of suprabasal epithelium to basal cells (by similarity).

• Plays a role in stabilizing the protein levels of desmosome plaque components DSP, JUP, PKP1, PKP2, and PKP3. Related references: PMID:22294297

• Involved in mechanotransduction: required for YAP1 localization at the plasma membrane in keratinocytes in response to mechanical strain via a complex with PKP1 and YWHAG, and positively regulates YAP1 target gene transcription and cell proliferation. Related references: PMID:31835537

• Positively regulates cellular contractility and cell junction formation via organization of cortical F-actin bundles and anchoring of actin to tight junctions, in conjunction with RAC1. Related references: PMID:22796473

• The cytoplasmic pool of DSG3 is required for CDH1 and CTNNB1 localization at developing adherens junctions, potentially via modulation of SRC activity; also inhibits keratinocyte migration by suppressing p38MAPK signaling, moderating wound healing. Related references: PMID:22294297 PMID:26763450

• Subcellularly, DSG3 localizes to the cell membrane, desmosomes, tight junctions, and cytoplasm. It is expressed in the basal and spinous layers of epidermis, skin, mucosa, esophageal mucosa, hair follicle outer root sheath, tongue, and tonsil.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 50–615 of human DSG3, covering the extracellular cadherin repeat domains; this design may contribute to specific recognition of native protein conformations.
• For IHC-P, standard antigen retrieval protocols (e.g., heat-induced epitope retrieval in citrate buffer, pH 6.0) may be applied; always include appropriate positive and negative tissue controls.
• For ELISA, the recombinant immunogen protein can serve as a tool for assay optimization and as a positive control; perform endpoint titration to determine optimal antibody dilution.
• This antibody reacts with human DSG3; cross-reactivity with other desmoglein or cadherin family members has not been fully characterized — independent validation is recommended for multiplex panels.
• The predicted molecular weight of DSG3 is approximately 108 kDa; if the antibody is adapted for Western blotting (not currently validated), consider using low-percentage gels and extended transfer times.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and research laboratories by providing a streamlined source for IVD raw materials, including antibodies against cell adhesion targets such as Desmoglein-3. Our portfolio encompasses validated polyclonal and monoclonal antibodies, matched antibody pairs, and bulk ancillary reagents, helping you accelerate assay development for autoimmune blistering diseases, epithelial biology, and beyond. For specialized requirements, we offer sourcing services for rare targets and customized production to bridge the gap from concept to clinic.