Anti-DSG2 Rabbit Polyclonal Antibody for WB, ELISA - Q14126

Core Product Specifications and Parameters

Biological Background: Desmoglein-2 Function and Localization

• Key component of desmosome cell-cell junctions, required for positive regulation of cellular adhesion and maintenance of desmosomal integrity. Related references: PMID:17559062 PMID:38395410
• Maintains pluripotency by regulating epithelial-to-mesenchymal transition (EMT) via interaction with and sequestration of CTNNB1 at cell contacts, reducing its nuclear translocation and target gene transcription. Related references: PMID:29910125
• Promotes pluripotency and multi-lineage differentiation potential of hematopoietic stem cells and mediates endothelial cell sprouting and elongation through junctional actin-CDHS association. Related references: PMID:27338829
• Limits inflammatory infiltration and apoptotic response in kidney tubular epithelial cells, contributing to epithelial barrier maintenance. Related references: PMID:38395410
• Acts as a positive modulator of CSK and EGFR activation by sequestering them away from lipid rafts, and disrupts CAV1 localization to lipid rafts; this function is independent of desmosomal junctions. Related references: PMID:26918609
• Localizes primarily at the cell membrane and desmosomes, but also found in the cytoplasm and secreted forms.
• Expressed in a wide range of tissues including undifferentiated pluripotent stem cells, cardiomyocytes, endothelial cells, pancreatic islets, and kidney tubular epithelia; expression patterns vary with differentiation stage.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the cytoplasmic region (aa 635-935), which may influence recognition of different isoforms or post-translationally modified forms; consider this when selecting detection methods.
• For Western blot, a prominent band near 122 kDa is expected in human cell or tissue lysates known to express DSG2, such as cardiac, endothelial, or stem cell lines.
• For ELISA, assess optimal coating concentrations and pair with appropriate capture/detection antibodies for sandwich assay development.
• Validate the antibody in relevant biological systems based on target expression; cardiac, cancer, or adhesion models are suitable starting points.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a streamlined path to IVD raw materials, technical support, and custom sourcing. For targets like DSG2—central to cell adhesion, cardiomyopathy, and oncology—we offer validated polyclonal and monoclonal antibodies, optimized antibody pairs, and bulk ancillary reagents. Our supply network can help secure rare-target raw materials and accelerate development from concept to clinic.