Anti-DSG1 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - Q02413

Core Product Specifications and Parameters

Biological Background: DSG1 Function and Localization

• Component of desmosome cell-cell junctions, positively regulating cellular adhesion (PubMed:19717567). Related references: PMID:19717567
• Promotes differentiation of keratinocytes by interacting with ERBIN, reducing RAS/ERK signaling through SHOC2 competition (PubMed:23524970). Related references: PMID:23524970
• Mediates interaction between desmosome and COP9 signalosome, promoting keratinocyte differentiation via deneddylation of EGFR and reducing EGFR stabilization (PubMed:28891468). Related references: PMID:28891468
• Critical for epidermal integrity and skin barrier function (By similarity).
• Required for esophageal epithelial barrier integrity and correct intercellular spacing (PubMed:24220297). Related references: PMID:24220297
• Required for recovery of epidermal architecture and correct differentiation marker expression following UVB exposure (PubMed:24594668). Related references: PMID:24594668
• Subcellularly located at cell membrane, desmosome, cytoplasm, and nucleus.
• Tissue specificity: expressed in all suprabasal epidermal layers, highest in granular layer, and in suprabasal esophageal epithelium (PubMed:24220297). Related references: PMID:24220297

Experimental Guidance and Technical Tips

• This polyclonal antibody, raised against a fragment corresponding to amino acids 800–1049 of human DSG1, may be suitable for Western blot (predicted band ~114 kDa), immunofluorescence on paraffin-embedded sections (IF-P), and ELISA.
• For Western blot, consider using reducing conditions and validate detection in your sample system, given reported cross-reactivity with both human and rat DSG1.
• For IF-P, antigen retrieval may be required; optimize based on your tissue and fixation protocol.
• As with any polyclonal antibody, lot-to-lot consistency should be verified for critical applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop IVD raw-material sourcing partner, providing diagnostic manufacturers and research institutes with access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw materials. This anti-DSG1 antibody supports research into desmosomal adhesion, keratinocyte differentiation, and skin barrier function, aligning with our comprehensive portfolio for dermatology and cell junction studies. From concept to clinic, we deliver the critical raw materials and technical expertise needed to accelerate your diagnostic development.