Anti-DSC1 Polyclonal Antibody for WB, ELISA - Q08554

Core Product Specifications and Parameters

Biological Background: DSC1 Function and Localization

• DSC1 (Desmocollin-1) is a calcium-dependent cadherin family member and a core component of desmosome cell-cell junctions.
• It positively regulates cell adhesion and is required for strong adhesion between granular keratinocytes in the epidermis.
• DSC1 moderates epidermal proliferation and differentiation, thereby maintaining postnatal epidermal barrier function and normal hair follicle morphology.
• The protein is strongly expressed in the epidermis, with lower expression detected in lymph node and tongue.
• Subcellularly, DSC1 localizes to the cell membrane at desmosomal cell junctions.
• Post-translational modifications include cleavage on a pair of basic residues and phosphorylation; it binds calcium and is glycosylated.
• Alternative splicing produces multiple isoforms; the canonical isoform has an approximate molecular weight of 100 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an intracellular region (amino acids 480–690) of human DSC1, suggesting the antibody likely recognizes the cytoplasmic domain.
• For Western blot, consider using reducing conditions and load lysates from epidermal or skin tissues; expect a band near 100 kDa, but isoforms or cleavage products may appear.
• For ELISA, the antibody may be used to detect recombinant or native DSC1; optimize coating and detection conditions for human, mouse, or rat samples.
• Validate antibody specificity by comparing signal in tissues with known DSC1 expression, such as epidermis (high) versus lymph node (low).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials. This anti-DSC1 polyclonal antibody exemplifies our commitment to supplying reliable reagents for epidermal barrier and cell adhesion research. Beyond antibodies, we offer validated antibody pairs, bulk ancillary reagents, and sourcing support for rare targets—helping you move from concept to clinic efficiently.