Anti-DRP1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O00429

Core Product Specifications and Parameters

Biological Background: DRP1 (Dynamin-1-like protein) Function and Localization

• DRP1, encoded by the DNM1L gene, is a dynamin-related GTPase essential for mitochondrial and peroxisomal division, mediating membrane fission through oligomerization (PubMed:11514614, PubMed:12499366, PubMed:17301055, PubMed:23530241, PubMed:23584531, PubMed:33850055). Related references: PMID:11514614 PMID:12499366 PMID:17301055 PMID:23530241 PMID:23584531 PMID:33850055
• Recruitment to mitochondrial scission sites is facilitated by MFF, MIEF1, and MIEF2 receptors, and DRP1 activity is dynamically regulated by phosphorylation, notably at Ser-637, which controls circadian ATP production (PubMed:23283981, PubMed:23921378, PubMed:29899447, PubMed:29478834). Related references: PMID:23283981 PMID:23921378 PMID:29899447 PMID:29478834
• Beyond mitochondrial fission, DRP1 localizes to peroxisomes, the Golgi apparatus, clathrin-coated pits, synaptic vesicles, and the cytosol, reflecting its roles in endocytosis and organelle dynamics (PubMed:17015472, PubMed:23792689, PubMed:9570752). Related references: PMID:17015472 PMID:23792689 PMID:9570752
• DRP1 is required for normal apoptosis, facilitating cytochrome c release and caspase activation, and is also essential for programmed necrosis execution (PubMed:22265414). Related references: PMID:22265414
• The protein is ubiquitously expressed, with highest levels in skeletal muscle, heart, kidney, and brain; multiple isoforms show tissue-specific expression, including a brain-specific isoform 1 (from UniProt TISSUE SPECIFICITY).
• Post-translational modifications, including phosphorylation, acetylation, S-nitrosylation, and ubiquitination, fine-tune DRP1 function (UniProt KEYWORDS).
• During mitosis, DRP1-mediated mitochondrial fission ensures proper organelle inheritance, and it plays a critical role in neuronal survival and brain development, particularly in Purkinje cells (PubMed:19411255, PubMed:17460227, PubMed:26992161, PubMed:27145208). Related references: PMID:19411255 PMID:17460227 PMID:26992161 PMID:27145208
• Overexpression of DRP1 inhibits peroxisomal division, while its rhythmic activity governed by circadian phosphorylation is vital for metabolic homeostasis (PubMed:29478834). Related references: PMID:29478834

Experimental Guidance and Technical Tips

• The immunogen (aa 411-710) covers the C-terminal variable domain and GTPase effector domain (GED) of human DRP1; as a polyclonal antibody, it may recognize multiple epitopes within this region.
• For Western blotting, expect a predominant band around 82 kDa; validate specificity using appropriate knockdown or knockout controls in your sample system.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval methods and fixation conditions; tissue staining should reflect the known mitochondrial and peroxisomal localization.
• This antibody cross-reacts with mouse and rat, making it suitable for rodent model studies; consider testing in relevant tissues such as brain, muscle, and heart.
• ELISA applications can use this antibody as a capture or detection reagent; perform checkerboard titrations to determine optimal working concentrations.

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CamelBio is your dedicated IVD raw-material sourcing partner, providing high-quality antibodies like this anti-DRP1 polyclonal antibody to accelerate mitochondrial dynamics and apoptosis research. We offer validated polyclonal and monoclonal antibodies, bulk ancillary reagents, and tailored procurement for rare targets. Contact us to explore customized solutions and technical support for your next diagnostic or research project.